Results 81 to 90 of about 8,447,464 (395)

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Costimulatory Molecules CD80/86 Trigger Non-Specific Cytotoxic Cell of Nile tilapia (Oreochromis niloticus) to Kill CIK Cells

Fishes, 2022
The teleost non-specific cytotoxic cell (NCC), as the evolutionary precursors of NK cells, is an important cytotoxic cell population in the innate immune system of teleost.
Yu Huang, Zhengsi Chen, Ruitao Xie, Kwaku Amoah, Bei Wang, Jia Cai, Yishan Lu, Jichang Jian   +7 more
doaj   +1 more source

Sustained synchronized neuronal network activity in a human astrocyte co-culture system [PDF]

, 2016
Impaired neuronal network function is a hallmark of neurodevelopmental and neurodegenerative disorders such as autism, schizophrenia, and Alzheimer's disease and is typically studied using genetically modified cellular and animal models.
Brône, Bert, Cik, Miroslav, De Vos, Winnok, Detrez, Jan R, Diels, Annick, Kuijlaars, Jacobine, Meert, Theo, Meneghello, Giulia, Nuydens, Rony, Oyelami, Tutu, Peeters, Pieter J, Rohrbacher, Jutta, Tuefferd, Marianne, Verheyen, An, Verschuuren, Marlies, Verstraelen, Peter, Versweyveld, Sofie   +16 more
core   +1 more source

Scaffolds for 3D Cell Culture and Cellular Agriculture Applications Derived From Non-animal Sources

Frontiers in Sustainable Food Systems, 2019
For decades, two-dimensional cell culture has been regarded as a major tool in cellular and molecular biology due to its simplicity, reproducibility and reliable nature.
Santiago Campuzano, A. Pelling
semanticscholar   +1 more source

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

Endothelial dysfunction due to the inhibition of the synthesis of nitric oxide: Proposal and characterization of an in vitro cellular model

Frontiers in Physiology, 2022
The vascular endothelium plays a pivotal role in the maintenance of vascular homeostasis, mediated by vasoactive molecules produced by endothelial cells. The balance between vasoconstrictor and vasodilator biomolecules is what guarantees this equilibrium.
Fernanda Cardoso da Silva, Fernanda Cardoso da Silva, Bruna Juber de Araújo, Bruna Juber de Araújo, Carina Santos Cordeiro, Vinícius Marques Arruda, Bruno Quintanilha Faria, Joyce Ferreira Da Costa Guerra, Thaise Gonçalves De Araújo, Cristina Ribas Fürstenau   +9 more
doaj   +1 more source

Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model. [PDF]

, 2017
In vitro experiments are essential to understand biological mechanisms; however, the gap between monolayer tissue culture and human physiology is large, and translation of findings is often poor.
Baar, Keith, Lee-Barthel, Ann, West, Daniel WD   +2 more
core   +2 more sources

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Cloning, characterization and expression analysis of caspase-8 genes from the golden pompano (Trachinotus ovatus)

Frontiers in Marine Science, 2022
Bacterial infection triggers apoptosis and immunity by regulating the expression of caspases. In this article, we report two caspase-8 paralogs, termed ToCas8 and ToCas8-like, from an economically important teleost golden pompano (Trachinotus ovatus ...
Fan Da, Xiaoju Wan, Guixiang Lin, Jichang Jian, Jichang Jian, Shuanghu Cai, Shuanghu Cai   +6 more
doaj   +1 more source

Axonal regeneration in hippocampal and spinal cord organotypic slice cultures [PDF]

, 2010
Under normal conditions, axonal regeneration after lesions is not possible in mature CNS but can occur in embryonic and early postnatal nervous systems. In recent years, a number of possible strategies to enhance axonal regeneration and eventually treat ...
Bonnici, Brenda
core   +1 more source