Results 41 to 50 of about 53,985,043 (260)

Survival-Span Method: How to Qualitatively Estimate Lifespan to Improve the Study of Aging, and not Disease, in Aging Studies

Frontiers in Aging, 2021
Lifespan analyses are important for advancing our understanding of the aging process. There are two major issues in performing lifespan studies: 1) late-stage animal lifespan analysis may include animals with non-terminal, yet advanced illnesses, which ...
Julia Adelöf, Jaime M. Ross, Jaime M. Ross, Madeleine Zetterberg, Madeleine Zetterberg, Malin Hernebring, Malin Hernebring   +6 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Toward an integrated ethical review process: an animal-centered research framework for the refinement of research procedures

Frontiers in Veterinary Science
The involvement of animals in research procedures that can harm them and to which they are deemed unable to consent raises fundamental ethical dilemmas.
Eleonora Nannoni, Clara Mancini
doaj   +1 more source

A Handful of Details to Ensure the Experimental Reproducibility on the FORCED Running Wheel in Rodents: A Systematic Review

Frontiers in Endocrinology, 2021
A well-documented method and experimental design are essential to ensure the reproducibility and reliability in animal research. Experimental studies using exercise programs in animal models have experienced an exponential increase in the last decades ...
Daniel Garrigos, Daniel Garrigos, Marta Martínez-Morga, Marta Martínez-Morga, Angel Toval, Angel Toval, Yevheniy Kutsenko, Yevheniy Kutsenko, Alberto Barreda, Alberto Barreda, Bruno Ribeiro Do Couto, Bruno Ribeiro Do Couto, Bruno Ribeiro Do Couto, Fernando Navarro-Mateu, Fernando Navarro-Mateu, Fernando Navarro-Mateu, Fernando Navarro-Mateu, José Luis Ferran, José Luis Ferran   +18 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Animal Research, Accountability, Openness and Public Engagement: Report from an International Expert Forum [PDF]

, 2019
In November 2013, a group of international experts in animal research policy (n = 11) gathered in Vancouver, Canada, to discuss openness and accountability in animal research.
Ormandy, Elisabeth H., Weary, Daniel M., Fisher, Mark, Cvek, Katarina, Rose, Margaret, Rowan, Andrew, Herrmann, Kathrin, Michael McDonald, Kathrin Herrmann, von Keyserlingk, Marina A.G., Marina A.G. von Keyserlingk, Mark Fisher, Milsom, William, William Milsom, Pru Hobson-West, Margaret Rose, Hobson-West, Pru, von Keyserlingk, Marina A. G., Andrew Rowan, Elisabeth H. Ormandy, Daniel M. Weary, McDonald, Michael, Joanne Zurlo, Zurlo, Joanne, Katarina Cvek   +24 more
core   +1 more source

Gluconate-Lactobionate-Dextran Perfusion Solutions Attenuate Ischemic Injury and Improve Function in a Murine Cardiac Transplant Model

Cells, 2022
Static cold storage is the cheapest and easiest method and current gold standard to store and preserve donor organs. This study aimed to compare the preservative capacity of gluconate-lactobionate-dextran (Unisol) solutions to histidine-tryptophan ...
Yinan Guo, Franka Messner, Sarah E. Beck, Marcos Iglesias Lozano, Hubert Schwelberger, Yichuan Zhang, Kai Kammers, Byoung Chol Oh, Elizabeth D. Greene, Gerald Brandacher, Kelvin G. M. Brockbank   +10 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

A Proposal for a Noxious Stimuli-Free, Moderate-Intensity Treadmill Running Protocol to Improve Aerobic Performance in Experimental Research on Rats

Metabolites
Background/Objectives: Animal models can help understand human physiological responses, including the response to exercise and physical activity. However, many of these models incorporate noxious stimuli for various scientific purposes.
Gilmara Gomes de Assis, Elda Olivia Nobre de Souza, Paulo Francisco de Almeida-Neto, Halil İbrahim Ceylan, Nicola Luigi Bragazzi   +4 more
doaj   +1 more source