Results 141 to 150 of about 384,231 (258)

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Accelerated Antibody Discovery Targeting the SARS-CoV-2 Spike Protein for COVID-19 Therapeutic Potential [PDF]

Antib Ther, 2021
Mullen TE, Abdullah R, Boucher J, Brousseau AS, Dasuri NK, Ditto NT, Doucette AM, Emery C, Gabriel J, Greamo B, Patil KS, Rothenberger K, Stolte J, Souders CA.   +13 more
europepmc   +2 more sources

Agonist antibody discovery: Experimental, computational, and rational engineering approaches. [PDF]

Drug Discov Today, 2022
Schardt JS, Jhajj HS, O'Meara RL, Lwo TS, Smith MD, Tessier PM.   +5 more
europepmc   +1 more source

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Advances in Therapeutic Antibody Discovery and Development Targeting G Protein-Coupled Receptors. [PDF]

Pharmacol Res Perspect
Hutchings CJ, Ünsal S, Rappas M, Barnes M, Waldhoer M, Holland B, Culka M.   +6 more
europepmc   +1 more source

A SARS-CoV-2 neutralizing antibody discovery by single cell sequencing and molecular modeling. [PDF]

J Biomed Res, 2022
Wang Z, Tang Q, Liu B, Zhang W, Chen Y, Ji N, Peng Y, Yang X, Cui D, Kong W, Tang X, Yang T, Zhang M, Chang X, Zhu J, Huang M, Feng Z.   +16 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Contrastive learning enables epitope overlap predictions for targeted antibody discovery. [PDF]

Patterns (N Y)
Holt CM, Janke AK, Amlashi P, Jamieson PJ, Marinov TM, Georgiev IS.   +5 more
europepmc   +1 more source

From structure to sequence: Antibody discovery using cryoEM. [PDF]

Sci Adv, 2022
Antanasijevic A, Bowman CA, Kirchdoerfer RN, Cottrell CA, Ozorowski G, Upadhyay AA, Cirelli KM, Carnathan DG, Enemuo CA, Sewall LM, Nogal B, Zhao F, Groschel B, Schief WR, Sok D, Silvestri G, Crotty S, Bosinger SE, Ward AB.   +18 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source