Results 51 to 60 of about 1,140,100 (305)

Antibody-antigen complex formation with immobilized immunoglobulins [PDF]

, 1992
We have investigated the complex formation between an immobilized monoclonal antibody and antigens that differ in size about 50-fold. As a model system, we used an iodinated progesterone derivative and a progesterone-horseradish peroxidase conjugate as ...
Se-Hwan Paek, Schramm, Willfried, Willfried Schramm, Paek, Se-Hwan   +3 more
core   +1 more source

Effect of Trastuzumab–HER2 Complex Formation on Stress-Induced Modifications in the CDRs of Trastuzumab

Frontiers in Chemistry, 2022
Asparagine deamidation and aspartic acid isomerization in the complementarity determining regions (CDRs) of monoclonal antibodies may alter their affinity to the target antigen.
Baubek Spanov, Victoria Aboagye, Oladapo Olaleye, Natalia Govorukhina, Nico C. van de Merbel, Nico C. van de Merbel, Rainer Bischoff   +6 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Antibody response of patients after postexposure rabies vaccination with small intradermal doses of purified chick embryo cell vaccine or purified Vero cell rabies vaccine

Bulletin of the World Health Organization
Although the introduction of tissue culture vaccines for rabies has dramatically improved the immunogenicity and safety of rabies vaccines, they are often prohibitively expensive for developing countries.
D.J. Briggs, A. Banzhoff, U. Nicolay, S. Sirikwin, B. Dumavibhat, S. Tongswas, C. Wasi   +6 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Plasmodium falciparum merozoite surface protein 2: epitope mapping and fine specificity of human antibody response against non-polymorphic domains [PDF]

, 2014
Two long synthetic peptides representing the dimorphic and constant C-terminal domains of the two allelic families of Plasmodium falciparum merozoite surface proteins 2 are considered promising malaria vaccine candidates.
Kajava, Andrey V., Diakité, Mahamadou, Felger, Ingrid, Servis, Catherine, Corradin, Giampietro, D’Alessandro, Alba, Moret, Remy, Balam, Saidou, Nebie, Issa, Kajava, Andrey V, Spertini, François, Olugbile, Sope, Smith, Thomas, D'Alessandro, Alba, Frank, Geraldine, Tanner, Marcel   +15 more
core   +1 more source

Recipient priming to one RBC alloantigen directly enhances subsequent alloimmunization in mice

Blood Advances, 2018
: Individuals that become immunized to red blood cell (RBC) alloantigens can experience an increased rate of antibody formation to additional RBC alloantigens following subsequent transfusion. Despite this, how an immune response to one RBC immunogen may
Seema R. Patel, Ashley Bennett, Kathryn Girard-Pierce, Cheryl L. Maier, Satheesh Chonat, Connie M. Arthur, Patricia E. Zerra, Amanda Mener, Sean R. Stowell   +8 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Generation and application of recombinant antibody fragments for prostate cancer detection. [PDF]

, 2010
Prostate cancer (PCa) is the most prevalent adenocarcinoma and the second highest cause of cancer-related deaths in men. Early diagnosis is required to identify the development of PCa to reduce the risk of the disease metastasising to different regions ...
Hayes, Conor
core