Results 51 to 60 of about 31,015 (230)

A Concept for Selection of Codon-Suppressor tRNAs Based on Read-Through Ribosome Display in an In Vitro Compartmentalized Cell-Free Translation System

Journal of Nucleic Acids, 2012
Here is presented a concept for in vitro selection of suppressor tRNAs. It uses a pool of dsDNA templates in compartmentalized water-in-oil micelles.
Atsushi Ogawa, Masayoshi Hayami, Shinsuke Sando, Yasuhiro Aoyama   +3 more
doaj   +1 more source

Future of the Genetic Code

Life, 2017
The methods for establishing synthetic lifeforms with rewritten genetic codes comprising non-canonical amino acids (NCAA) in addition to canonical amino acids (CAA) include proteome-wide replacement of CAA, insertion through suppression of nonsense codon,
Hong Xue, J. Tze-Fei Wong
doaj   +1 more source

Forced Ambiguity of the Leucine Codons for Multiple-Site-Specific Incorporation of a Noncanonical Amino Acid. [PDF]

PLoS ONE, 2016
Multiple-site-specific incorporation of a noncanonical amino acid into a recombinant protein would be a very useful technique to generate multiple chemical handles for bioconjugation and multivalent binding sites for the enhanced interaction.
Inchan Kwon, Eun Sil Choi
doaj   +1 more source

Biallelic mutations in valyl-tRNA synthetase gene VARS are associated with a progressive neurodevelopmental epileptic encephalopathy. [PDF]

, 2019
Aminoacyl-tRNA synthetases (ARSs) function to transfer amino acids to cognate tRNA molecules, which are required for protein translation. To date, biallelic mutations in 31 ARS genes are known to cause recessive, early-onset severe multi-organ diseases ...
Accogli, Andrea, Ben-Omran, Tawfeg, Bernard, Geneviève, Chowdhury, Shimul, Crawford, John R, Dimmock, David, Friedman, Jennifer, Gleeson, Joseph G, Guerrero, Kether, Hildreth, Amber, Issa, Mahmoud Y, James, Kiely N, Kingsmore, Stephen F, Koehler, Alanna E, Lo, Wing-Sze, Mendes, Marisa I, Musaev, Damir, Nahas, Shareef, Omar, Tarek EI, Salomons, Gajja S, Smith, Desiree E, Stanley, Valentina, Tran, Luan T, Wang, Rengang, Wigby, Kristen, Wright, Meredith S, Xu, Zhiwen, Zaki, Maha S, Zhai, Liting   +28 more
core   +2 more sources

Mistranslating the genetic code with leucine in yeast and mammalian cells

RNA Biology
Translation fidelity relies on accurate aminoacylation of transfer RNAs (tRNAs) by aminoacyl-tRNA synthetases (AARSs). AARSs specific for alanine (Ala), leucine (Leu), serine, and pyrrolysine do not recognize the anticodon bases.
Josephine Davey-Young, Farah Hasan, Rasangi Tennakoon, Peter Rozik, Henry Moore, Peter Hall, Ecaterina Cozma, Julie Genereaux, Kyle S. Hoffman, Patricia P. Chan, Todd M. Lowe, Christopher J. Brandl, Patrick O’Donoghue   +12 more
doaj   +1 more source

Crystal structure of 70S ribosome with both cognate tRNAs in the E and P sites representing an authentic elongation complex. [PDF]

PLoS ONE, 2013
During the translation cycle, a cognate deacylated tRNA can only move together with the codon into the E site. We here present the first structure of a cognate tRNA bound to the ribosomal E site resulting from translocation by EF-G, in which an entire L1
Shu Feng, Yun Chen, Yong-Gui Gao
doaj   +1 more source

tRNA splicing [PDF]

, 1998
Introns interrupt the continuity of many eukaryal genes, and therefore their removal by splicing is a crucial step in gene expression. Interestingly, even within Eukarya there are at least four splicing mechanisms.
Abelson, John, Li, Hong, Trotta, Christopher R.   +2 more
core   +1 more source

Effects of tRNA modification on translational accuracy depend on intrinsic codon–anticodon strength

Nucleic Acids Research, 2015
Cellular health and growth requires protein synthesis to be both efficient to ensure sufficient production, and accurate to avoid producing defective or unstable proteins.
Nandini Manickam, Kartikeya Joshi, Monika J Bhatt, P. Farabaugh   +3 more
semanticscholar   +1 more source

Base-pairing versatility determines wobble sites in tRNA anticodons of vertebrate mitogenomes.

PLoS ONE, 2012
BackgroundVertebrate mitochondrial genomes typically have one transfer RNA (tRNA) for each synonymous codon family. This limited anticodon repertoire implies that each tRNA anticodon needs to wobble (establish a non-Watson-Crick base pairing between two ...
Miguel M Fonseca, Sara Rocha, David Posada   +2 more
doaj   +1 more source

Conservation of an intricate circuit for crucial modifications of the tRNAPhe anticodon loop in eukaryotes

RNA: A publication of the RNA Society, 2015
Post-transcriptional tRNA modifications are critical for efficient and accurate translation, and have multiple different roles. Lack of modifications often leads to different biological consequences in different organisms, and in humans is frequently ...
Michael P Guy, E. Phizicky
semanticscholar   +1 more source