Results 81 to 90 of about 15,481 (256)

Detection of Antibody-Antigen Reactions using Surface Acoustic Wave and Electrochemical Immunosensors

, 2004
Traditional immunoassays for the detection of specific antibody-antigen reactions are expensive, time-consuming and non-trivial. In this work, immunosensors based on surface acoustic wave and electrochemical techniques are presented, which provide ...
Luo, Cheng-Ping
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Antibody Conjugates via Disulfide Bridging: Towards therapeutic and diagnostic applications

, 2015
Antibodies play a prominent role in chemical and biological research and the largest application of chemical bioconjugation reagents is in the production of antibody conjugates.
Hull, EA
core  

Adaptation of the Ultrasensitive HIV-1 p24 Antigen Assay to Dried Blood Spot Testing. [PDF]

, 2007
Implementation of molecular tests for the assessment of pediatric HIV-1 infection in resource-limited countries is difficult because of technical complexity and costs.
Speck, R F, Böni, J, Jullu, B, Schüpbach, J, Knuchel, M C, Mshinda, H, Schüpbach, Jörg, Nadal, D, Stoeckle, Marcel P, Tanner, M, Böni, Jürg, Shah, Cyril, Jullu, Boniphace, Speck, Roberto F, Shah, C, Tomasik, Z, Stoeckle, M P, Nadal, David, Tomasik, Zuzana, Mshinda, Hassan, Knuchel, Marlyse C, Tanner, Marcel   +21 more
core   +1 more source

Identifying gene expression signatures for risk stratification of postoperative adjuvant chemotherapy in colorectal cancer

FEBS Open Bio, EarlyView.
A novel signature integrating genome‐wide analysis with clinical factors predicts recurrence in stage II colorectal cancer and enables a new risk stratification to guide postoperative adjuvant chemotherapy. Clinical risk stratification for postoperative recurrence in patients with pathological stage II (pStage II) colorectal cancer (CRC) is essential ...
Mayuko Otomo, Keisuke Okuno, Shuichi Watanabe, Sakiko Oba, Hiroyasu Kagawa, Masanori Tokunaga, Daisuke Ban, Yusuke Kinugasa   +7 more
wiley   +1 more source

Futtermittel-spezifisches IgG und IgE vor und nach Eliminationsdiäten bei allergischen Hunden [PDF]

, 2012
Serum food allergen-specific antibody testing is widely offered to identify suitable ingredients for diets to diagnose adverse food reaction (AFR) in dogs with allergic skin disease.
Zimmer, Anja
core  

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

In-vitro demonstration of cell-mediated immunity to vaccinia virus in man [PDF]

, 1975
Cell mediated immunity to vaccinia virus in man was studied by lymphocyte transformation. Vaccinia antigen, propagated on BHK-21 and Vero cells, could be used successfully for in-vitro testing after partial purification as well as crude infectious ...
Koszinowski, Ulrich H., Thomssen, R., Volkmann, B.   +2 more
core  

Deriving a germinal center lymphocyte migration model from two-photon data [PDF]

, 2008
Recently, two-photon imaging has allowed intravital tracking of lymphocyte migration and cellular interactions during germinal center (GC) reactions. The implications of two-photon measurements obtained by several investigators are currently the subject ...
Michael Meyer-Hermann, Garin, A, Marie Kosco-Vilbois, Figge, MT, Figge, Marc Thilo, Marc Thilo Figge, Meyer-Hermann, M, Kosco-Vilbois, M, Kosco-Vilbois, Marie, Gunzer, Matthias, Garin, Alexandre, Kai-Michael Toellner, Meyer-Hermann, Michael (Prof. Dr.), Alexandre Garin, Gunzer, M, Matthias Gunzer, Toellner, Kai-Michael   +16 more
core   +1 more source