Results 1 to 10 of about 7,472 (116)

pH-dependent activation of the Na+/H+ antiporter NhaA and conformational dynamics of its N-terminus [PDF]

Nature Communications
Na⁺/H⁺ antiporters are vital for regulating intracellular pH and sodium ion levels across all domains of life. In Escherichia coli, NhaA is the principal Na⁺/H⁺ antiporter, exhibiting strong pH sensitivity and rapid turnover, yet the structural ...
Tsai-Hsuan Weng, Balázs Fábián, Elena Olkhova, Sonja Welsch, Sarah Luise Schmidt, Tsafi Danieli, Yael Keren, Abraham Rimon, Schara Safarian, Gerhard Hummer, Etana Padan, Hartmut Michel   +11 more
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The evolution of a Na+-sensitive Vibrio cholerae mutant unmasks the moonlighting aminopeptidase PepA as a regulator of nhaB Na+/H+ antiporter gene expression [PDF]

Journal of Bacteriology
The pathogenic bacterium Vibrio cholerae is aquatic and can be cultivated in nutrient media with increased salt concentration. To maintain osmotic balance, V. cholerae uses Na+/H+ antiporters and a Na+-translocating NADH:quinone oxidoreductase (Na+-NQR).
Sebastian Herdan, Katharina Kohm, Robert Warneke, Florian Roth, Nadja Glaser, Lucia Villani, Nele Rüping, Lea Dreher, Nicolas Görge, Trung Duc Hoang, Emina Schunke, Claudia C. Häse, Juri Rappsilber, Günter Fritz, Fabian M. Commichau, Johannes Gibhardt, Julia Steuber   +16 more
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The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach

Biomolecular Concepts, 2022
In the present work, we discuss the way in which the parallel application of the patch-clamp technique and the 2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) fluorescence detection for recording luminal proton changes allows the functional ...
Antonella Gradogna, Armando Carpaneto, Jose M. Pardo   +2 more
exaly   +2 more sources

Homeostats: The hidden rulers of ion homeostasis in plants [PDF]

Quantitative Plant Biology
Ion homeostasis is a crucial process in plants that is closely linked to the efficiency of nutrient uptake, stress tolerance and overall plant growth and development.
Ingo Dreyer, Naomí Hernández-Rojas, Yasnaya Bolua-Hernández, Valentina de los Angeles Tapia-Castillo, Sadith Z. Astola-Mariscal, Erbio Díaz-Pico, Franko Mérida-Quesada, Fernando Vergara-Valladares, Oscar Arrey-Salas, María E. Rubio-Meléndez, Janin Riedelsberger, Erwan Michard   +11 more
doaj   +2 more sources

Mutagenesis and functional analysis of SotB: A multidrug transporter of the major facilitator superfamily from Escherichia coli

Frontiers in Microbiology, 2022
Dysfunction of the major facilitator superfamily multidrug (MFS Mdr) transporters can lead to a variety of serious diseases in human. In bacteria, such membrane proteins are often associated with bacterial resistance.
Guoqing Zhai, Zhengyu Zhang, Changjiang Dong   +2 more
doaj   +1 more source

Diverse Functions of Plant Zinc-Induced Facilitator-like Transporter for Their Emerging Roles in Crop Trait Enhancement

Plants, 2021
The major facilitator superfamily (MFS) is a large and diverse group of secondary transporters found across all kingdoms of life. Zinc-induced facilitator-like (ZIFL) transporters are the MFS family members that function as exporters driven by the ...
Varsha Meena, Shivani Sharma, Gazaldeep Kaur, Bhupinder Singh, Ajay Kumar Pandey   +4 more
doaj   +1 more source

Do Amino Acid Antiporters Have Asymmetric Substrate Specificity?

Biomolecules, 2023
Amino acid antiporters mediate the 1:1 exchange of groups of amino acids. Whether substrate specificity can be different for the inward and outward facing conformation has not been investigated systematically, although examples of asymmetric transport ...
Gregory Gauthier-Coles, Stephen J. Fairweather, Angelika Bröer, Stefan Bröer   +3 more
doaj   +1 more source

Ins and Outs of Rocker Switch Mechanism in Major Facilitator Superfamily of Transporters

Membranes, 2023
The major facilitator superfamily (MFS) of transporters consists of three classes of membrane transporters: symporters, uniporters, and antiporters. Despite such diverse functions, MFS transporters are believed to undergo similar conformational changes ...
Stephanie Sauve, Joseph Williamson, Adithya Polasa, Mahmoud Moradi   +3 more
doaj   +1 more source

Ectopic expression of GmNHX3 and GmNHX1, encoding two Glycine max Na+/H+ vacuolar antiporters, improves water deficit tolerance in Arabidopsis thaliana

Biologia Plantarum, 2021
The importance of Na+/H+ antiporters in salt tolerance in plants has been demonstrated in many studies, but much less is known about their protective role during drought stress. To study their possible contribution to water deficit tolerance, two closely
E.M. PARDO, L. TOUM, L.S. PÉREZ-BORROTO, L. FLEITAS, J.P. GALLINO, S. VIDAL MACHI, A. VOJNOV, A.P. CASTAGNARO, B. WELIN   +8 more
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Functional Roles of the Conserved Amino Acid Sequence Motif C, the Antiporter Motif, in Membrane Transporters of the Major Facilitator Superfamily

Biology, 2023
The biological membrane surrounding all living cells forms a hydrophobic barrier to the passage of biologically important molecules. Integral membrane proteins called transporters circumvent the cellular barrier and transport molecules across the cell ...
Manuel F. Varela, Anely Ortiz-Alegria, Manjusha Lekshmi, Jerusha Stephen, Sanath Kumar   +4 more
doaj   +1 more source