Results 261 to 270 of about 47,774 (288)
Some of the next articles are maybe not open access.
Antisense oligonucleotides: strategies for delivery
Pharmaceutical Science & Technology Today, 1998Abstract The development of antisense oligonucleotides as therapeutic agents has progressed significantly over the past 10–15 years. In order for antisense oligodeoxynucleotides to become effective therapeutic agents, improvement in the delivery and distribution of these compounds must occur.
Keith J Miller, Sudip K Das
openaire +1 more source
In vitro transport and delivery of antisense oligonucleotides
2000A variety of techniques are currently available to enhance the cellular uptake and pharmacological effectiveness of antisense oligonucleotides in the in vitro setting. The choice of technique will depend on the context of investigation, the likelihood of cytotoxity due to the delivery agents, and the ease and convenience of the approach.
J, Hughes +7 more
openaire +2 more sources
Effective intracellular delivery of oligonucleotides in order to make sense of antisense
Journal of Controlled Release, 2004 For more than two decades, antisense oligonucleotides (ODNs) have been used to modulate gene expression for the purpose of applications in cell biology and for development of novel sophisticated medical therapeutics.
Dick Hoekstra
exaly +2 more sources
PAMAM Dendrimers as Delivery Agents for Antisense Oligonucleotides
Pharmaceutical Research, 1999To investigate the potential use of PAMAM dendrimers for the delivery of antisense oligonucleotides into cells under conditions that mimic the in vivo environment.We used HeLa cells stably transfected with plasmid pLuc/705 which has a luciferase gene interrupted by a human beta-globin intron mutated at nucleotide 705, thus causing incorrect splicing ...
H, Yoo, P, Sazani, R L, Juliano
openaire +2 more sources
Comparison of antisense oligonucleotide drug delivery systems
Journal of Controlled Release, 2004Antisense oligonucleotides (AS-ONs) are specific drugs to inhibit gene expression at the transcriptional level. They possess a poor bioavailability and can be degraded by nucleases very rapidly. Therefore, a strong need for the development of oligonucleotide drug delivery systems exists. In the present study, two commercially available liposomes (DOTAP,
Jörg, Weyermann +2 more
openaire +2 more sources
Liposomes as a Drug Delivery System for Antisense Oligonucleotides
Antisense Research and Development, 1992Antisense oligonucleotides seem to provide a promising new tool for the therapy of viral diseases and of cancer. However, before the therapeutic potential of antisense compounds can be fulfilled, it will be necessary to overcome significant problems relating to their inefficient uptake by cells and their rapid loss from the body. Phospholipid vesicles (
R L, Juliano, S, Akhtar
openaire +2 more sources
Oral delivery of siRNA and antisense oligonucleotides
Journal of Drug Targeting, 2009Inhibition of gene expression with antisense oligonucleotides or RNA interference (RNAi) mediated gene silencing by small interfering RNA (siRNA) has tremendous potential to silence the expression of disease-causing genes in the clinic. A major hurdle to their widespread clinical use is the safe and efficient delivery to target cells in vivo.
openaire +2 more sources
Targeted Lipid Nanoparticles for Antisense Oligonucleotide Delivery
Current Pharmaceutical Biotechnology, 2014Antisense oligonucleotides (AS-ODNs) are short, single-stranded DNA molecules designed to bind specifically to a target messenger RNA (mRNA) and down-regulate gene expression. Despite being a promising class of therapeutics for a variety of diseases, they face major hurdles limiting their clinical application, including low intracellular delivery and ...
Raquel, Petrilli +4 more
openaire +2 more sources
Antisense Oligonucleotide Delivery with Polyhexylcyanoacrylate Nanoparticles as Carriers
Methods, 1999Polyalkylcyanoacrylate nanoparticles are effective colloidal drug carriers and were prepared by an emulsion polymerization process. Antisense oligonucleotides were loaded on the particles by adsorption. A cationic polymer, DEAE-dextran, was incorporated into the particle matrix or a cationic hydrophobic detergent (CTAB) was used to form a lipophilic ...
openaire +2 more sources
Cell Delivery and Mechanisms of Action of Antisense Oligonucleotides
1993Publisher Summary This chapter discusses the synthetic nucleic acids interfering with gene expression (SNAIGE), which are being developed as specific regulators of gene expression both for fundamental studies and/or for potential (medical) applications. Although straightforward in principle, the approach still faces many unknowns.
Leonetti, Jean Paul +4 more
openaire +2 more sources