Results 251 to 260 of about 94,605 (281)
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Seminars in cancer biology, 1992
Growing evidence indicates that antisense oligodeoxynucleotides can specifically inhibit gene expression thereby providing an essential tool for understanding gene function and the potential to affect abnormal cell proliferation. Because oncogene activation is intimately involved in tumour initiation and progression, down-regulation of oncogene ...
CALABRETTA, Bruno, Skorski T, Zon G.
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Growing evidence indicates that antisense oligodeoxynucleotides can specifically inhibit gene expression thereby providing an essential tool for understanding gene function and the potential to affect abnormal cell proliferation. Because oncogene activation is intimately involved in tumour initiation and progression, down-regulation of oncogene ...
CALABRETTA, Bruno, Skorski T, Zon G.
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Antisense oligonucleotides as therapeutic agents
Journal of Cellular Physiology, 1999Antisense oligonucleotides can block the expression of specific target genes involved in the development of human diseases. Therapeutic applications of antisense techniques are currently under investigation in many different fields. The use of antisense molecules to modify gene expression is variable in its efficacy and reliability, raising objections ...
GALDERISI, Umberto +2 more
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Therapeutic implications of antisense oligonucleotides
International Journal of Clinical & Laboratory Research, 1992Antisense oligonucleotides and their derivatives have been shown to be specific inhibitors of gene expression. They are considered a very promising new generation of drugs, potentially useful in most human diseases, including cancers and viral infections.
D. Lattuada +3 more
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Purification of antisense oligonucleotides
2000Chromatography is an effective tool for obtaining high-purity synthetic oligonucleotides for a variety of end uses, including antisense drug therapy. Reversed-phase and anion-exchange chromatographies are widely used techniques for this application. While selectivity of these techniques can be modified by methods such as ion-pair RP-HPLC or affinity ...
R R, Deshmukh, D L, Cole, Y S, Sanghvi
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Mini-antisense Oligonucleotides
Nucleosides and Nucleotides, 1997Abstract A new strategy of selective DNA target modification was proposed. The using of reactive derivatives of short oligonucleotides in the presence of flanking effector pair allows one to modify DNA target only when the perfect complementary complex of DNA target and oligonucleotide tandem is formed.
D. V. Pyshnyi +4 more
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Conjugated Antisense Oligonucleotides
Nucleosides and Nucleotides, 1997Abstract We have employed chemical modification strategies to improve cellular ahsorption of oligonucleotides. These include the conjugation of various pendant moieties to the oligonucleotide to affect its overall physical properties such as hydrophobicity, charge, and amphipathicity as well as pendants that may mediate absorption by binding to certain
Muthiah Manoharan +11 more
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Antisense oligonucleotides in cutaneous therapy
Pharmacology & Therapeutics, 2001Antisense oligonucleotides have been the subject of intense interest as research tools to elucidate the functions of gene products and as therapeutic agents. Initially, their mode of action was poorly understood and the biological effects of oligonucleotides were often misinterpreted.
C J, Wraight, P J, White
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Antisense Oligonucleotides: Promise and Reality
Annual Review of Pharmacology and Toxicology, 2001Antisense oligonucleotides have been used for more than a decade to downregulate gene expression. Phosphodiester oligonucleotides are nuclease sensitive, and the more nuclease-resistant phosphorothioate oligonucleotides are now in common use in the laboratory and have entered clinical trials.
I, Lebedeva, C A, Stein
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History of Antisense Oligonucleotides
2003Biological science is a rapidly flowing experimental stream, at times encountering a dam that impedes further progress. At such a pomt, a single crack may induce a major breakthrough Discovery of the double helical structure of DNA in 1953 (1) caused such an event, with flooding of new information into the area now known as molecular biology.
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Antisense Oligonucleotides as Research Tools
2003The use of antisense oligonucleotides as both research tools and therapeutic molecules has emerged as a powerful alternative to small molecule inhibitors. Antisense oligonucleotides are short pieces of chemically modified DNA designed to hybridize to specific mRNA sequences present in the target gene.
J K, Taylor, S R, Cooper, N M, Dean
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