Evolution and biogeography of apple stem grooving virus [PDF]
Background Apple stem grooving virus (ASGV) has a wide host range, notably including apples, pears, prunes and citrus. It is found worldwide. Method In this study, two near complete genomes, and seven coat protein (CP) sequences of Iranian isolates from ...
Shohreh Shokri +3 more
doaj +5 more sources
First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain [PDF]
Loquat (Eriobotrya japonica) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was
Celia Canales +3 more
doaj +6 more sources
First report of apple stem grooving virus infection in loquat from India. [PDF]
Loquat, commonly known as Eriobotrya japonica, is a major subtropical fruit from the Rosaceae family that is native to China but also found in most of Europe and Asia, including India. Apple stem grooving virus (ASGV) infecting loquat was detected using leaf samples collected from Himachal Pradesh (India) through DAC-ELISA followed by RT-PCR assays ...
Suman R +5 more
europepmc +3 more sources
The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars [PDF]
The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultivars of a 25 years old orchard.
Jiban Kumar Kundu
doaj +2 more sources
Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR [PDF]
Apple stem grooving virus (ASGV) is a destructive viral pathogen of pome fruit trees that causes significant losses to fruit production worldwide.
Sung-Woong Kim +3 more
doaj +2 more sources
Distribution of Apple stem grooving virus in apple trees in the Czech Republic [PDF]
From 1997 to 2000 the distribution of Apple stem grooving virus (ASGV) in selected intensive apple orchards was investigated at six sites in the Czech Republic. Detection of ASGV was by ELISA.
Jaroslav Polák, Jiřina Zieglerová
doaj +2 more sources
Evaluation of Reference Genes for the Relative Quantification of Apple stem grooving virus and Apple mosaic virus in Apple Trees. [PDF]
A SYBR Green(®)-based one step RT-qPCR assay was developed for the detection and quantification of Apple stem grooving virus (ASGV) and Apple mosaic virus (ApMV). The RT-qPCR assay employed seven plant-expressed genes-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), 18S ribosomal RNA, ubiquitin, ribosomal protein S19, Rubisco, RNA polymerase subunit ...
Gadiou S, Kundu JK.
europepmc +4 more sources
The reverse transcription polymerase chain reaction (RT-PCR) was successfully used to determine the occurrence of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in field-grown apple cultivars.
Jiban Kumar Kundu
doaj +2 more sources
Detection of Apple stem grooving virus in different tissues of apple trees throughout the year [PDF]
The suitability of different apple tissues for Apple stem grooving virus (ASGV) detection throughout the year was checked by RT-PCR and ELISA. Detectable amounts of ASGV were generally found in all tissues (bark, dormant buds, petals and leaves) tested ...
Jiban Kumar. Kundu +2 more
doaj +2 more sources
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification [PDF]
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for
Nam-Yeon Kim +5 more
doaj +2 more sources

