Results 91 to 100 of about 585,125 (273)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Femto-Laser Processed Metasurface With Fano Response: Applications to a High Performance Refractometric Sensor

Photonic Sensors
The practical development of compact modern nanophotonic devices relies on the availability of fast and low-cost fabrication techniques applicable to a wide variety of materials and designs.
Mahmoud H. Elshorbagy, Alejandro San-Blas, Luis Miguel Sanchez-Brea, Santiago M. Olaizola, Jesús del Hoyo, Angela Soria-Garcia, Joaquin Andres-Porras, Verónica Pastor-Villarrubia, Javier Alda   +8 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Avoided crossing resonances: structural and dynamical aspects

, 2009
We examine structural and dynamical properties of quantum resonances associated with an avoided crossing and identify the parameter shifts where these properties attain maximal or extreme values, first at a general level, and then for a two-level system ...
Hernandez-Concepcion, E., Lizuain, I., Muga, J. G.   +2 more
core   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Elastic scattering losses in the four-wave mixing of Bose Einstein Condensates

, 2004
We introduce a classical stochastic field method that accounts for the quantum fluctuations responsible for spontaneous initiation of various atom optics processes. We assume a delta-correlated Gaussian noise in all initially empty modes of atomic field.
Bach R, Bonifacio R, Bonifacio R, Chwede?czuk J, Drummond P D, Gardiner C W, Goldstein E V, Goldstein E V, Goldstein E V, Haake F, Harris S E, J Chwedeńczuk, Javanainen J, K Rzazewski, M Trippenbach, Magde D, Mandel L, Mostowski J, Mostowski J, Perina J, Poulsen U V, Raymer M G, Trippenbach M, Trippenbach M, Vardi A, Yariv A, Yurovsky V A, Zin P Chwede?czuk J Perez A Trippenbach M Rza??ewski K   +27 more
core   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Understanding bio‐based polymers: A study of origins, properties, biodegradation and their impact on health and the environment

FEBS Open Bio, EarlyView.
This review provides an overview of bio‐based polymer sources, their unique functional properties and their environmental impact, and addresses their role as sustainable alternatives. It discusses end‐of‐life options, including composting and anaerobic digestion for renewable energy.
Sabina Kolbl Repinc, Blaž Stres, Mirica Karlovits, Igor Karlovits, Petra Jerič, Ondrej Panák, Anja Verbič, Blaž Likozar, Uroš Novak   +8 more
wiley   +1 more source

Parameter optimization for MINFLUX microscopy enabled single particle tracking

Communications Biology
MINFLUX fluorescence microscopy is a recently introduced super-resolution approach for studying cellular structures and their dynamics with highest detail. MINFLUX (MFX) performs Single Particle Tracking (SPT) at runtime. The ad hoc signal interpretation
Bela T. L. Vogler, Giovanni De Angelis, Ziliang Zhao, Christian Eggeling, Francesco Reina   +4 more
doaj   +1 more source