Results 111 to 120 of about 55,142 (236)
Development of a Photostable pH Biosensor Based on mStayGold
The front cover shows the iterative directed evolution process used to evolve the biosensor. Incubation with CO2 allowed for direct screening of both fluorescence and pH sensitivity in mutant colonies on agar. Our efforts produced serapH1.0, a photostable pH biosensor that is highly fluorescent at physiologically neutral pH (7.4) and dim at acidic pH ...
Michael Chang +7 more
wiley +1 more source
Acid Sulfite Pulping of Hemp: Effects of Processing Conditions on Lyocell Pulp Quality
Industrial hemp is emerging as a promising renewable source for next‐generation textile fibers. This study evaluates whether acid sulfite pulping can produce hemp‐based dissolving pulp suitable for lyocell manufacturing. While the process is historically linked to increased cellulose accessibility, it did not achieve the low lignin content and ...
Lelia Lawson +3 more
wiley +1 more source
A Comparative Biochemical Study of Oleate Hydratases
Four oleate hydratases (Ohys) from diverse homologous families (HFams) were characterized side‐by‐side in terms of expression levels, oligomeric state, flavin adenine dinucleotide (FAD) affinity, thermostability, and activity. A general favorable effect of the reduction of FAD to FADH2 for Ohy activity was demonstrated, with (R)‐10‐hydroxystearic acid (
Maxim van Delft +3 more
wiley +1 more source
This study broadens our understanding of the role of intramolecular interactions in molecular recognition and highlights the importance of considering their formation when designing artificial receptors, new drugs, and related systems. Aminoalcohols represent an important class of compounds and serve as building blocks for numerous biologically active ...
Manuel Stapf, Monika Mazik
wiley +1 more source
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson +6 more
wiley +1 more source
Engineered Optogenetic Circuits In Yeast with Self‐Sustained Outputs
Optogenetic quorum‐sensing (OptoQS) circuit enables Saccharomyces cerevisiae to record transient light stimulus with self‐sustained outputs. Panel a indicates the design of the OptoQS circuit based on G‐protein coupled receptor (GPCR) signaling cascade. Panel b indicates the mode of action of surrogate messenger‐mediated signal transmission at the cell
Cong Fan +7 more
wiley +1 more source
This study firstly isolated a tuna‐derived gut probiotic Acinetobacter seifertii YFT067. Dietary administration of YFT067 played significant roles in promoting growth performance, SCFAs production, lipid absorption, and metabolism of the host, indicating YFT067 as a promising probiotic candidate for enhancing tuna aquaculture productivity through ...
Ying Zou +4 more
wiley +1 more source
The bioactive substances we extracted from Lycium barbarum residue (LBR) have a typical polysaccharide structure. When 1.80% LBR was used to replace maize in the diet, the average daily gain of rossbred Simmental was significantly increased, and anti‐inflammatory ability was improved. In addition, LBR improved the fecal microbial composition, increased
Kun Cai +10 more
wiley +1 more source
To control expression of the synthetic replication operon in the orthogonal replication system, this step uses anarabinose-induced dCas9–sgRNA repression systemto reduce downstream operon component expression, therebyimproving orthogonal replicon transformation efficiency.
openaire +1 more source
A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm +3 more
wiley +1 more source

