Results 131 to 140 of about 103,313 (260)
To control expression of the synthetic replication operon in the orthogonal replication system, this step uses anarabinose-induced dCas9–sgRNA repression systemto reduce downstream operon component expression, therebyimproving orthogonal replicon transformation efficiency.
openaire +1 more source
Multi‐Targeting Non‐Specific Genome Engineering in Bacteria
In this study, we provide the first case to use the multi‐targeting integrase (MTI) systems in bacteria and develop a host‐independent generalizable approach, MNGE (Multi‐targeting Non‐specific Genome Engineering), for multi‐copy and random integration of metabolic genes or pathways in both Gram‐positive and Gram‐negative bacteria, which will ...
Runze Sun +7 more
wiley +1 more source
Structural studies of a fucogalactoxyloglucan from pinus radiata primary cell walls : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]
1. The changes in carbohydrate composition of elongating Pinus radiata primary cell walls were investigated. In the hemicellulose B extracts, a large increase in the percentage of non-starch, non-cellulosic, glucose was found to occur on cessation of ...
Little, John William Lester
core
Aggregation of Modified Glucuronoxylan in Water and DMSO
Neutron scattering study of dispersibility of glucuronoxylan and modified glucuronoxylan, in water and dimethyl sulfoxide (DMSO). ABSTRACT Glucuronoxylans are known to be only partly soluble in aqueous media. Chemical modification often aims to improve solubility, yet observations of aggregation even of the modified xylans are not uncommon.
Chonnipa Palasingh +7 more
wiley +1 more source
Dual-topology insertion of a dual-topology membrane protein. [PDF]
Some membrane transporters are dual-topology dimers in which the subunits have inverted transmembrane topology. How a cell manages to generate equal populations of two opposite topologies from the same polypeptide chain remains unclear.
Bowie, James U +2 more
core
ABSTRACT This study demonstrates a robust chemometric framework for high‐throughput compositional analysis of lignocellulosic biomass using a portable near‐infrared (NIR) spectrometer. Sugarcane bagasse solids produced by pressurized solvent fractionation (PSF) under batch and continuous conditions were analyzed to predict cellulose, hemicellulose, and
Julia M. M. Caetano +7 more
wiley +1 more source
Repurpose an FDA‐Approved Antibody Using DARPin‐Scaffolded Bridge Protein
The cell line and scalable upstream/downstream process development needed for every new monoclonal antibody are both highly expensive and time‐consuming, significantly increasing the cost of new antibody therapeutics. By contrast, the production of many synthetic binders can be accomplished rapidly and with lower production costs.
Michael Woolley +4 more
wiley +1 more source
ABSTRACT Fungal polysaccharides (FPs) represent a diverse class of bioactive macromolecules widely studied for their nutritional, therapeutic, and biotechnological value. This review synthesizes recent advances (2020–2025) in the structural characterization, biological functions, and translational potential of FPs.
Muhammad Aaqil +6 more
wiley +1 more source

