Optogenetic Cancer Therapy Using the Light-Driven Outward Proton Pump Rhodopsin Archaerhodopsin-3 (AR3). [PDF]
J Am Chem SocMedicines used for cancer treatment often cause serious side effects by damaging normal cells due to nonspecific diffusion. To address this issue, we previously developed an optical method to induce apoptotic cell death via intracellular pH ...
Nakao S +6 more
europepmc +2 more sources
Redshifted and Near‐infrared Active Analog Pigments Based upon Archaerhodopsin‐3 [PDF]
Photochemistry and Photobiology, 2019 Archaerhodopsin‐3 (AR3) is a member of the microbial rhodopsin family of hepta‐helical transmembrane proteins, containing a covalently bound molecule of all‐trans retinal as a chromophore. It displays an absorbance band in the visible region of the solar
S. Ganapathy +6 more
semanticscholar +5 more sources
Function of Bacterioruberin in Archaerhodopsin 4, from Expression to Characterization [PDF]
Biophysical Journal, 2017 Chao Sun +9 more
semanticscholar +3 more sources
Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter [PDF]
Science Advances, 2021 Photoactivated genetically encoded voltage indicators (GEVIs) have the potential to enable optically sectioned voltage imaging at the intersection of a photoactivation beam and an imaging beam. We developed a pooled high-throughput screen to identify archaerhodopsin mutants with enhanced photoactivation. After screening ~10
Miao‐Ping Chien +9 more
openalex +5 more sources
A voltage-dependent fluorescent indicator for optogenetic applications, archaerhodopsin-3: Structure and optical properties from in silico modeling [PDF]
F1000Research, 2017 It was demonstrated in recent studies that some rhodopsins can be used in optogenetics as fluorescent indicators of membrane voltage. One of the promising candidates for these applications is archaerhodopsin-3.
Dmitrii M. Nikolaev +4 more
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Two substates in the O intermediate of the light-driven proton pump archaerhodopsin-2.
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2022 The proton pumping cycle of archaerhodopsin-2 (aR2) was investigated over a wide pH range and at different salt concentrations. We have found that two substates, which are spectroscopically and kinetically distinguishable, occur in the O intermediate.
T. Kouyama, K. Ihara
semanticscholar +3 more sources
Strongly Hydrogen Bonded Water Networks Detected in the Archaerhodopsin-3 Proton Pump [PDF]
Biophysical Journal, 2012 We examined structural changes of the protein, retinal chromophore and internal water molecules during the photocycle of Archaerhodopsin-3 (AR3), a light-driven proton pump found in Halobrubrum sodemense. This protein, which belongs to the archael rhodopsin family, has a ∼75% sequence homology with the more extensively studied bacteriorhodopsin (BR ...
John I. Ogren +5 more
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Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor Archon2. [PDF]
Int J Mol Sci, 2020 Archon2 is a fluorescent voltage sensor derived from Archaerhodopsin 3 (Arch) of Halorubrum sodomense using robotic multidimensional directed evolution approach. Here we report absorption and emission spectroscopic studies of Archon2 in Tris buffer at pH
Penzkofer A, Silapetere A, Hegemann P.
europepmc +2 more sources
In Situ Study of the Function of Bacterioruberin in the Dual-Chromophore Photoreceptor Archaerhodopsin-4. [PDF]
Angewandte Chemie International Edition, 2018 While certain archaeal ion pumps have been shown to contain two chromophores, retinal and the carotenoid bacterioruberin, the functions of bacterioruberin have not been well explored.
Chao Sun +6 more
semanticscholar +3 more sources
Archaerhodopsin Variants with Enhanced Voltage Sensitive Fluorescence in Mammalian and Caenorhabditis elegans Neurons [PDF]
Nature Communications, 2014 Probing the neural circuit dynamics underlying behaviour would benefit greatly from improved genetically encoded voltage indicators. The proton pump Archaerhodopsin-3 (Arch), an optogenetic tool commonly used for neuronal inhibition, has been shown to ...
Nicholas C. Flytzanis +8 more
semanticscholar +4 more sources