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International Journal of Biological Macromolecules, 2017
A library of Pseudoalteromonas carrageenovora arylsulfatase mutants was constructed by introducing random mutagenesis using error-prone PCR. After screening, one mutant strain was obtained whose arylsulfatase had improved thermal stability. Protein sequence analysis revealed one amino acid substitution of H260L.
Yanbing Zhu +6 more
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A library of Pseudoalteromonas carrageenovora arylsulfatase mutants was constructed by introducing random mutagenesis using error-prone PCR. After screening, one mutant strain was obtained whose arylsulfatase had improved thermal stability. Protein sequence analysis revealed one amino acid substitution of H260L.
Yanbing Zhu +6 more
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Arylsulfatase A in pseudodeficiency
Human Genetics, 1984Arylsulfatase A (ASA) is found to be deficient in healthy individuals (pseudo arylsulfatase A deficiency) who usually show in vitro ASA levels in the range of metachromatic leukodystrophy patients. The in vitro properties of ASA in pseudodeficiency were studied in cultured fibroblasts.
Barbara Herz, Gideon Bach
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Deficient glycosylation of arylsulfatase A in pseudo arylsulfatase-A deficiency
Molecular and Cellular Biochemistry, 1990Deficient arylsulfatase-A activity is diagnostic of a neurodegenerative human lysosomal storage disease, metachromatic leukodystrophy. Paradoxically, similar enzyme deficiency also occurs in normal individuals, who are known as being pseudo arylsulfatase-A deficient. We showed previously that this phenotype is associated with a structural gene mutation
Mohammed Ameen +5 more
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Arylsulfatase A pseudodeficiency in Chinese
Human Genetics, 1996Arylsulfatase A (ASA) pseudodeficiency was found to be much rarer in Taiwan than in most western countries (2.5% versus 7.3%-20% carrier rate). The linkage of two mutations (A2725G and A1788G) in the pseudodeficiency allele was preserved in Chinese, and A2725G did not occur alone.
Wuh-Liang Hwu +5 more
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A Km mutant of arylsulfatase A
Clinica Chimica Acta, 1982Abstract The apparent Michealis constant for p-nitrocatechol sulfate of arylsulfatase A of fibroblasts of a metachromatic leukodystrophy patient has been determined and compared to that of normal controls. Control cells gave an apparent Km of 0.7 mmol/l, while the apparent Km of patient's cells was determined to be 19.8 mmol/l.
F. A. Hommes +3 more
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Diagnosis of arylsulfatase A deficiency
American Journal of Medical Genetics, 1992AbstractMetachromatic leukodystrophy (MLD) is a neurologically devastating autosomal recessive disorder in humans associated with deficient arylsulfatase A activity. However, clinically normal individuals described as being pseudo‐arylsulfatase‐A deficient also demonstrate the same deficiency.
Patricia L. Chang +2 more
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The purification of arylsulfatases A and B
Biochemical Medicine, 1984Abstract Arylsulfatases A and B (arylsulfate sulfohydrolase, EC 3.1.6.1) are isoenzymatical. Both enzymes are located mainly in the liver, though they are widely distributed in mammalian tissues [rabbit liver (1), ox liver (2), human kidney (3), human brain (4), and human liver (5)].
Kazuo Ohuchi +3 more
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Structure of the arylsulfatase A gene
European Journal of Biochemistry, 1990A 14‐kb genomic clone containing the entire gene of human lysosomal arylsulfatase A was isolated. The arylsulfatase A gene is about 3.2 kb long and has eight exons (103–320 nucleotides in size). All intron–exon splice junctions conformed to the GT/AG consensus sequence.
J. Kreysing +2 more
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