Results 131 to 140 of about 6,281 (181)

A Km mutant of arylsulfatase A

Clinica Chimica Acta, 1982
Abstract The apparent Michealis constant for p-nitrocatechol sulfate of arylsulfatase A of fibroblasts of a metachromatic leukodystrophy patient has been determined and compared to that of normal controls. Control cells gave an apparent Km of 0.7 mmol/l, while the apparent Km of patient's cells was determined to be 19.8 mmol/l.
A M, Harben, N, Krawiecki, R, Marcus, F A, Hommes   +3 more
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The purification of arylsulfatases A and B

Biochemical Medicine, 1984
Abstract Arylsulfatases A and B (arylsulfate sulfohydrolase, EC 3.1.6.1) are isoenzymatical. Both enzymes are located mainly in the liver, though they are widely distributed in mammalian tissues [rabbit liver (1), ox liver (2), human kidney (3), human brain (4), and human liver (5)].
T, Ozeki, M, Kan, K, Ohuchi, K, Iwaki
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Structure of the arylsulfatase A gene

European Journal of Biochemistry, 1990
A 14‐kb genomic clone containing the entire gene of human lysosomal arylsulfatase A was isolated. The arylsulfatase A gene is about 3.2 kb long and has eight exons (103–320 nucleotides in size). All intron–exon splice junctions conformed to the GT/AG consensus sequence.
J, Kreysing, K, von Figura, V, Gieselmann   +2 more
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Diagnosis of arylsulfatase A deficiency

American Journal of Medical Genetics, 1992
AbstractMetachromatic leukodystrophy (MLD) is a neurologically devastating autosomal recessive disorder in humans associated with deficient arylsulfatase A activity. However, clinically normal individuals described as being pseudo‐arylsulfatase‐A deficient also demonstrate the same deficiency.
Z G, Li, J S, Waye, P L, Chang
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Arylsulfatase of sea urchin sperm—Distribution of arylsulfatase in the gonads and gametes of echinoderms

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1979
1. Fairly high activities of arylsulfatase are found in the sperm and mature testes of all the sea urchins studied; Strongylocentrotus intermedius, Strongylocentrotus nudus, Hemicentrotus pulcherrimus and Anthocidaris crassispina, whereas the activities in the ovaries and eggs of these animals are low. 2.
T, Moriya, M, Hoshi
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Choroideremia with leukoencephalopathy and arylsulfatase A pseudodeficiency

Journal of the Neurological Sciences, 1996
A 33-year-old male patient was admitted to our hospital because of progressive gait disturbance and involuntary movement of the neck. He showed choroideremia, distal motor neuropathy, and leukoencephalopathy on T2-weighted brain magnetic resonance imaging (MRI).
W, Matsuyama, M, Kuriyama, M, Nakagawa, H, Kanazawa, S, Takenaga, S, Ijichi, M, Osame   +6 more
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Characterization of human arylsulfatase a glycans

International Journal of Biochemistry, 1994
Despite numerous studies on arylsulfatase A, the structure of the glycans present in each of its two subunits has not been determined. This is important because the carbohydrate component of human arylsulfatase A synthesized in tumor tissues and transformed cells has been shown to undergo apparent changes.
P, Laidler, A, Litynska, M, Galka-Walczak, B, Wojczyk   +3 more
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Specific determination of arylsulfatase A activity

Experientia, 1986
Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was ...
H, Inoue, Y, Seyama, S, Yamashita
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Studies on the charge isomers of arylsulfatase A

Biochemical Medicine, 1985
Human liver arylsulfatase A was resolved into six fractions by narrow pH range preparative isoelectric focusing. Analytical isoelectric focusing revealed that most enzyme fractions were composed of two adjacent charge isomers. Nevertheless, there was considerable enrichment of charge species which allowed a comparative study of selected properties ...
T A, Sarafian, K K, Tsay, W E, Jackson, A L, Fluharty, H, Kihara   +4 more
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The determination of arylsulfatases in biological fluids

Clinica Chimica Acta, 1970
Abstract A simple, highly sensitive method for the determination of arylsulfatases in serum, urine, saliva and other biological fluids is described. A procedure is given for the preparation of highly purified 4-methylumbelliferone sulfate, the substrate used in this assay.
H, Rinderknecht, M C, Geokas, C, Carmack, B J, Haverback   +3 more
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