\u3cem\u3eArabidopsis thaliana\u3c/em\u3e GLX2-1 Contains a Dinuclear Metal Binding Site, but Is Not a Glyoxalase 2 [PDF]
In an effort to probe the structure and function of a predicted mitochondrial glyoxalase 2, GLX2-1, from Arabidopsis thaliana, GLX2-1 was cloned, overexpressed, purified and characterized using metal analyses, kinetics, and UV–visible, EPR, and 1H-NMR ...
Bennett, Brian+3 more
core +2 more sources
Localization of arylsulfatase in Pseudomonas C12B [PDF]
Arylsulfatase was released almost completely from intact cells of Pseudomonas C12B after osmotic shock or after treatment with lysozyme. These results suggest that the enzyme is cell wall associated in this soil isolate.
J R George, J W Fitzgerald
openaire +3 more sources
Computational analysis of bacterial sulfatases and their modifying enzymes [PDF]
The sequence analysis of enzymes that might modify bacterial sulfatases should be useful in the task of identifying the human sulfatase-modifying homologs — enzymes that are defective in the rare inherited disease multi-sulfatase ...
Kolter, Roberto, Schirmer, Andreas
core +1 more source
Structural Insights into Endobiotic Reactivation by Human Gut Microbiome-Encoded Sulfatases [PDF]
Phase II drug metabolism inactivates xenobiotics and endobiotics through the addition of either a glucuronic acid or sulfate moiety prior to excretion, often via the gastrointestinal tract.
Creekmore, Benjamin C.+7 more
core +2 more sources
Non-Coordinative Binding of O2 at the Active Center of a Copper-Dependent Enzyme [PDF]
Molecular oxygen (O 2 ) is a sustainable oxidation reagent. O 2 is strongly oxidizing but kinetically stable and its final reaction product is water. For these reasons learning how to activate O 2 and how to steer its reactivity along desired reaction ...
Leisinger, Florian+2 more
core +1 more source
Development and validation of an extraction method based on dispersive liquid–liquid microextraction for the analysis by GC–MS of propofol in three different biological samples (blood, urine, and hair). The proposed method is very simple and rapid.
Sara Odoardi+4 more
wiley +1 more source
Cultural conditions of arylsulfatase activity in Escherichia coli [PDF]
Arylsulfatase activity and growth were estimated in Escherichia coli, isolated from marine sediment. Maximum activity was observed at pH 6.6 whereas the maximum growth was at pH 5.6.
Chandramohan, D.+2 more
core
Pleomorphic adenoma and carcinoma ex‐pleomorphic adenoma tumorigenesis: A proteomic analysis
Abstract Objectives To conduct a comprehensive proteomic analysis of normal salivary gland tissue, pleomorphic adenoma (PA), and carcinoma ex‐pleomorphic adenoma (CXPA), and validate the proteomic findings using immunohistochemistry. Methods Six normal salivary gland tissues, seven PA and seven CXPA samples underwent laser microdissection followed by ...
Virgílio Gonzales Zanella+13 more
wiley +1 more source
Riding the sulfur cycle - metabolism of sulfonates and sulfate esters in Gram-negative bacteria [PDF]
Sulfonates and sulfate esters are widespread in nature, and make up over 95% of the sulfur content of most aerobic soils. Many microorganisms can use sulfonates and sulfate esters as a source of sulfur for growth, even when they are unable to metabolize ...
Kertesz, Michael A.
core
Characterizing Diagnostic Delays in Metachromatic Leukodystrophy: A Real‐World Data Approach
ABSTRACT Neurodegeneration in metachromatic leukodystrophy (MLD) may be preceded by systemic complications. Characterization of these features is critical to define barriers to early diagnosis and treatment eligibility for gene therapy. We utilized medical billing (claims) datasets and a natural history study to capture pre‐diagnosis MLD‐related events.
Ali Mohajer+5 more
wiley +1 more source