Results 31 to 40 of about 464 (153)
Abstract Low‐chilling apple varieties are gaining attention in plain regions with high temperatures but viruses are also adapting to these conditions. In this report, we have characterized Apple stem grooving virus and Apple scar skin viroid from the low‐chilling apple samples collected from district District Mandla of Madhya Pradesh and Kangra of ...
A. Rani +6 more
wiley +1 more source
Field detection of multiple RNA viruses/viroids in apple using a CRISPR/Cas12a‐based visual assay
Summary Co‐infection of apple trees with several viruses/viroids is common and decreases fruit yield and quality. Accurate and rapid detection of these viral pathogens helps to reduce losses and prevent virus spread. Current molecular detection assays used for apple viruses require specialized and expensive equipment. Here, we optimized a CRISPR/Cas12a‐
Jian Jiao +11 more
wiley +1 more source
Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”
In order to breed virus‐free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture plantlets was detected via the real‐time reverse ...
Weimin Zhong +10 more
wiley +1 more source
Detection of Apple stem grooving virus in different tissues of apple trees throughout the year
The suitability of different apple tissues for Apple stem grooving virus (ASGV) detection throughout the year was checked by RT-PCR and ELISA. Detectable amounts of ASGV were generally found in all tissues (bark, dormant buds, petals and leaves) tested ...
Jiban Kumar. Kundu +2 more
doaj +1 more source
The reverse transcription polymerase chain reaction (RT-PCR) was successfully used to determine the occurrence of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in field-grown apple cultivars.
Jiban Kumar Kundu
doaj +1 more source
The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultivars of a 25 years old orchard.
Jiban Kumar Kundu
doaj +1 more source
Occurrence Status of Five Apple Virus and Viroid in Korea
The investigation of the infection rate of domestic apple orchards by four types of apple viruses (Apple chlorotic leaf spot virus [ACLSV], Apple stem pitting virus [ASPV], Apple stem grooving virus [ASGV], Apple mosaic virus [ApMV]) and one type of ...
Seongkyun Lee +6 more
doaj +1 more source
Virus-free and ASGV-infected plantlets for RNA-Seq.
(A) ASGV-infected in vitro grown plantlets. (B) Detection of Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple skin scar viroid (ASSVd) and Apple dimple fruit viroid (ADFVd) using RT-PCR ...
Ting Ye (346594) +7 more
core +1 more source
Abstract The Panel on Plant Health performed a listing of non‐EU viruses and viroids (reported hereinafter as viruses) of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L. and Vitis L. A systematic literature review identified 197 viruses infecting one or more of the host genera under consideration. Viruses were allocated
EFSA Panel on Plant Health (PLH) +26 more
wiley +1 more source
Gene coverage statistics of the two RNA-seq libraries: virus-free and ASGV-infected.
Gene coverage statistics of the two RNA-seq libraries: virus-free and ASGV-infected.
Ting Ye (346594) +7 more
core +1 more source

