Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation [PDF]
JCI Insight, 2021 T cell receptor (TCR) triggering by antigen results in metabolic reprogramming that, in turn, facilitates the exit of T cells from quiescence. The increased nutrient requirements of activated lymphocytes are met, in part, by upregulation of cell surface ...
Helen Carrasco Hope +5 more
doaj +6 more sources
Filamentation of asparagine synthetase in Saccharomyces cerevisiae. [PDF]
PLoS Genetics, 2018 Asparagine synthetase (ASNS) and CTP synthase (CTPS) are two metabolic enzymes crucial for glutamine homeostasis. A genome-wide screening in Saccharomyces cerevisiae reveal that both ASNS and CTPS form filamentous structures termed cytoophidia.
Shanshan Zhang +4 more
doaj +5 more sources
Asparagine Synthetase in Cancer: Beyond Acute Lymphoblastic Leukemia [PDF]
Frontiers in Oncology, 2020 Asparagine Synthetase (ASNS) catalyzes the synthesis of the non-essential amino acid asparagine (Asn) from aspartate (Asp) and glutamine (Gln). ASNS expression is highly regulated at the transcriptional level, being induced by both the Amino Acid ...
Martina Chiu +4 more
doaj +6 more sources
Human asparagine synthetase associates with the mitotic spindle [PDF]
Biology Open, 2018 Cancer cells are characterized by extensive reprogramming of metabolic pathways in order to promote cell division and survival. However, the growth promotion effects of metabolic reprogramming can be due to moonlighting functions of metabolic enzymes as ...
Chalongrat Noree +2 more
doaj +4 more sources
Cereal asparagine synthetase genes. [PDF]
Ann Appl Biol, 2021 AbstractAsparagine synthetase catalyses the transfer of an amino group from glutamine to aspartate to form glutamate and asparagine. The accumulation of free (nonprotein) asparagine in crops has implications for food safety because free asparagine is the precursor for acrylamide, a carcinogenic contaminant that forms during highâtemperature cooking and
Raffan S, Halford NG.
europepmc +4 more sources
Asparagine synthetase (ASNS) Drives Tumorigenicity in Small Cell Lung Cancer [PDF]
BiomedicinesObjectives: Small cell lung cancer (SCLC) is an aggressive neuroendocrine carcinoma characterized by rapid proliferation, early metastasis, and limited therapeutic response.
Minho Jeong +5 more
doaj +2 more sources
Cellular and molecular characterization of two novel asparagine synthetase gene mutations linked to asparagine synthetase deficiency. [PDF]
J Biol Chem, 2022 Asparagine synthetase (ASNS) catalyzes synthesis of asparagine (Asn) and Glu from Asp and Gln in an ATP-dependent reaction. Asparagine synthetase deficiency (ASNSD) results from biallelic mutations in the ASNS gene. Affected children exhibit congenital microcephaly, continued brain atrophy, seizures, and often premature mortality.
Staklinski SJ +9 more
europepmc +3 more sources
Effects of A6E Mutation on Protein Expression and Supramolecular Assembly of Yeast Asparagine Synthetase [PDF]
Biology, 2021 Asparagine synthetase deficiency (ASD) has been found to be caused by certain mutations in the gene encoding human asparagine synthetase (ASNS). Among reported mutations, A6E mutation showed the greatest reduction in ASNS abundance.
Thunyarat Surasiang, Chalongrat Noree
doaj +2 more sources
Metabolomic Profiling of Asparagine Deprivation in Asparagine Synthetase Deficiency Patient-Derived Cells. [PDF]
Nutrients, 2023 The natural amino acid asparagine (Asn) is required by cells to sustain function and proliferation. Healthy cells can synthesize Asn through asparagine synthetase (ASNS) activity, whereas specific cancer and genetically diseased cells are forced to obtain asparagine from the extracellular environment.
Chang MC +5 more
europepmc +4 more sources
Targeting Asparagine Synthetase in Tumorgenicity Using Patient-Derived Tumor-Initiating Cells [PDF]
Cells, 2022 Reprogramming of energy metabolism is regarded as one of the hallmarks of cancer; in particular, oncogenic RAS has been shown to be a critical regulator of cancer metabolism. Recently, asparagine metabolism has been heavily investigated as a novel target
Gen Nishikawa +7 more
doaj +2 more sources