Results 131 to 140 of about 11,688 (172)
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l-Asparagine synthetase: A marker for metastases
Biochemical Medicine, 1977Abstract Leukemia 5178 Y AR (resistant to l -asparaginase) contains l -asparagine synthetase (EC 6.3.5.4). As this neoplasm grows in the inguinal tissue of BDF1 mice, it metastasizes widely. l -Asparagine synthetase has been found to increase in concentration in liver, spleen, kidney, and several other tissues as a concomitant of such ...
R G, Cable, V D, King, D A, Cooney
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Immunochemical characterization of rat testicular asparagine synthetase
Archives of Biochemistry and Biophysics, 1992We studied immunochemical properties of rat testicular asparagine synthetase. Western blot analysis of testis extract with polyclonal antibody raised against purified asparagine synthetase revealed an immunoreactive band at 62 kDa. The pancreas, brain, thymus, and spleen also showed 62-kDa bands. The intensities of these bands were roughly proportional
S, Hongo +5 more
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A new assay for l-asparagine synthetase
Journal of Biochemical and Biophysical Methods, 1980A fast, relatively inexpensive method of measuring the enzymatic formation of L-asparagine from L-aspartate is presented. This radiochemical assay requires simple manipulations making it ideal for working with large numbers of samples, while maintaining high sensitivity and reproducibility.
C A, Luehr, S M, Schuster
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Guinea pig asparagine synthetase
Biochimica et Biophysica Acta (BBA) - Enzymology, 1969Abstract The partial purification and properties of guinea pig asparagine synthetase ( l -aspartate: ammonia ligase (AMP), EC 6.3.1.1) are described. The enzyme activity is present largely in the high-speed supernatant fractions and is found in extracts from all tissues with the highest specific activities in extracts from spleen, liver, intestine ...
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Asparagine synthetase from γ-irradiated potatoes
Archives of Biochemistry and Biophysics, 1969Abstract γ-Irradiation of potatoes at sprout-inhibiting dose was found to enhance the asparagine synthetase activity. This activation was dose-dependent and maximum activation was obtained between 5 and 25 krads; 10 krad being the optimum dose. Asparagine synthetase was purified to about 50-fold.
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[97] Asparagine synthetase (Tumor Cells)
1970Publisher Summary This chapter presents the assay, purification, and properties of asparagine synthetase from a strain of mouse leukemia. The enzyme preparation is incubated with L-aspartate-4- 14 C, L-glutamine, Mg 2+ , and adenosine triphosphate (ATP).
Bernard Horowitz +2 more
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Kinetic mechanism of beef pancreatic L-asparagine synthetase
Biochemistry, 1981The kinetic mechanism of bovine pancreatic asparagine synthetase was deduced from initial velocity studies and product inhibition studies of both the glutamine-dependent and ammonia-dependent reactions. For the glutamine-dependent pathway, parallel lines were observed in the double reciprocal plot of 1/V vs.
R S, Markin, C A, Luehr, S M, Schuster
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Regulation of mammalian asparagine synthetase by adenine nucleotides
Biochemical and Biophysical Research Communications, 1976Initial velocity kinetic data indicate that ADP and AMP are inhibitors of mammalian liver asparagine synthetase. The non-product nucleotide ADP is a much more potent inhibitor than AMP, although both apparently compete for the same site. This modifier site, however, does not overlap spatially with the substrate site for ATP.
F C, Wedler, K, Eismann
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Inhibition of l-asparagine synthetase by mineral cations
Toxicology and Applied Pharmacology, 1979The inhibition of l-asparagine synthetase from Leukemia 5178YAR and mouse pancreas by mineral cations was examined. Of the 24 agents tested in vitro, 5 (cadmium chloride, cupric chloride, mercuric chloride, mercurous chloride, and zinc chloride) inhibited the utilization of l-glutamine and NH4Cl by the enzymes by nearly 90% at a concentration of 1 mm ...
H A, Milman, D A, Cooney, J M, Ward
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Ethacrynic acid—an inhibitor of l-asparagine synthetase
Biochemical Pharmacology, 1975Abstract Ethacrynic acid, a clinically useful diuretic, has been shown to inhibit l -asparagine synthetase from leukemia 5178Y resistant to l -asparaginase (L5178Y/AR) in vitro. This inhibition is thought to involve the formation of an adduct between ethacrynic acid and sulfhydryl functions on the enzyme; the adduct is not readily reversible even ...
H N, Jayaram +5 more
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