Results 141 to 150 of about 11,688 (172)
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Purification and characterization of beef pancreatic asparagine synthetase
Archives of Biochemistry and Biophysics, 1985Bovine pancreatic asparagine synthetase has been partially purified using ammonium sulfate fractionation, DEAE ion-exchange, Cibacron Blue affinity chromatography, and HPLC anion-exchange chromatography to a specific activity of 170 nmol asparagine produced min-1 mg protein-1, or 1400-fold, from a crude homogenate.
C A, Luehr, S M, Schuster
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[96] Asparagine synthetase (Lactobacillus arabinosus)
1970Publisher Summary This chapter discusses the assay, purification, and properties of asparagine synthetase. Asparagine synthetase in the extracts of Lactobacillus arabinosus is most easily measured by substituting hydroxylamine for ammonia. The amount of β -aspartylhydroxamic acid formed is determined colorimetrically with ferric chloride reagent ...
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Kinetic mechanism of asparagine synthetase from Vibrio cholerae
Bioorganic Chemistry, 2004Asparagine synthetase B (AsnB) catalyzes the formation of asparagine in an ATP-dependent reaction using glutamine or ammonia as a nitrogen source. To obtain a better understanding of the catalytic mechanism of this enzyme, we report the cloning, expression, and kinetic analysis of the glutamine- and ammonia-dependent activities of AsnB from Vibrio ...
Vicente, Fresquet +3 more
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Multiple forms of rat liver L-asparagine synthetase
Biochemical and Biophysical Research Communications, 1979Abstract Extraction of rat liver asparagine synthetase from rats maintained on normal protein containing diets yields activity in a region corresponding to a molecular weight of 57,000. Decreasing the amount of protein in the diets caused the asparagine synthetase activity to appear in two forms, one being in the 57,000 region and the other in the ...
R S, Markin, S M, Schuster
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Measuring Asparagine Synthetase Activity in Crude Plant Extracts
Journal of Agricultural and Food Chemistry, 2000Asparagine synthetase B (AS) is the primary enzyme responsible for asparagine synthesis in plants. Routine biochemical studies of this enzyme's activity have been hindered by several problems including enzyme instability and rapid physiological turnover, endogenous inhibitors, competing pathways, and asparaginase activity.
J G, Romagni, F E, Dayan
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Role of pancreatic L-asparagine synthetase in homeostasis of L-asparagine.
American Journal of Physiology-Endocrinology and Metabolism, 1979L-Asparagine synthetase from mouse pancreas was found to be associated principally with the exocrine pancreas and to be dependent on the age of the animal, but not on gender, diet, or the presence of tumor under the conditions examined. The function of the pancreatic enzyme appears to be to supply L-asparagine for the synthesis of pancreatic proteins ...
H A, Milman, D A, Cooney, D M, Young
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Pediatric Neurology, 2016
Asparagine synthetase deficiency is an autosomal recessive neurometabolic disorder characterized clinically by severe congenital microcephaly, global developmental delay, intractable epilepsy, and motor impairment in the form of spastic quadriparesis. Diagnosis is confirmed by findings of low cerebral spinal fluid or plasma asparagine in addition to a ...
Muhammad Talal, Alrifai, Majid, Alfadhel
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Asparagine synthetase deficiency is an autosomal recessive neurometabolic disorder characterized clinically by severe congenital microcephaly, global developmental delay, intractable epilepsy, and motor impairment in the form of spastic quadriparesis. Diagnosis is confirmed by findings of low cerebral spinal fluid or plasma asparagine in addition to a ...
Muhammad Talal, Alrifai, Majid, Alfadhel
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Mechanistic issues in asparagine synthetase catalysis.
Advances in enzymology and related areas of molecular biology, 1998The enzymatic synthesis of asparagine is an ATP-dependent process that utilizes the nitrogen atom derived from either glutamine or ammonia. Despite a long history of kinetic and mechanistic investigation, there is no universally accepted catalytic mechanism for this seemingly straightforward carboxyl group activating enzyme, especially as regards those
N G, Richards, S M, Schuster
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Asparagine Synthetase in Zea mays
1979In legume cotyledons, asparagine is synthesized by a transfer of the amide nitrogen of glutamine to aspartate. The asparagine synthetase reaction requires ATP-Mg²⁺, aspartate and glutamine as substrates, although NH₄Cl can act as a rather inefficient nitrogen donor.
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