Results 41 to 50 of about 11,688 (172)

Inhibition of Plant Asparagine Synthetase by Monoterpene Cineoles [PDF]

Plant Physiology, 2000
Abstract Asparagine (Asn) synthetase (AS) is the key enzyme in Asn biosynthesis and plays an important role in nitrogen mobilization. Despite its important physiological function, little research has been done documenting inhibitors of plant AS.
J G, Romagni, S O, Duke, F E, Dayan
openaire   +2 more sources

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

ATF4‐mediated stress response as a therapeutic vulnerability in chordoma

Molecular Oncology, EarlyView.
We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone, James Dunford, Eleanor Calcutt, Vicki Gamble, Filiz Senbabaoglu Aksu, Lorena Ligammari, Georgina Wherry, Giorgia Gaeta, John C. Christianson, Adrienne M. Flanagan, Udo Oppermann, Adam P. Cribbs   +11 more
wiley   +1 more source

ETV4 Promotes Colorectal Cancer Progression by Reprogramming Asparagine Metabolism to Remodel the Stromal Microenvironment

Advanced Science, EarlyView.
ETV4 coordinates a dual transcriptional program by upregulating MET and asparagine synthetase (ASNS). The resulting asparagine (Asn) secretion acts as a metabolic signal, reprogramming hepatic stellate cells (HSCs) and primary cancer‐associated fibroblasts (CAFs) toward an inflammatory phenotype. These activated stromal cells release HGF to sustain the
Dujiang Fu, Meijia Zhang, Maoping Cai, Miao Wang, Ziao Huang, Zesheng Lin, Yixin Liu, Wang Qian, Guopeng Chen, Yuxing Liang, Dongyi Wei, Jing xie, Picheng Yan, Yuanyuan Qu, Yongchang Wei   +14 more
wiley   +1 more source

Functional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma

eLife, 2015
Current therapies for sarcomas are often inadequate. This study sought to identify actionable gene targets by selective targeting of the molecular networks that support sarcoma cell proliferation.
Simone Hettmer, Anna C Schinzel, Daria Tchessalova, Michaela Schneider, Christina L Parker, Roderick T Bronson, Nigel GJ Richards, William C Hahn, Amy J Wagers   +8 more
doaj   +1 more source

Alternating High‐Fat and Polysaccharide Diets Modulates Gut Phage‐Bacterial Interplay

Advanced Science, EarlyView.
This study reveals how alternating high‐fat and polysaccharide diets reshape the human gut virome and enhance phage‐bacteria interactions. Using large‐scale metagenomic meta‐analysis and a time‐resolved mouse model, the authors show that diets strongly modulate phage abundance, lifestyle, and gene exchange, offering new insights into nutrition‐guided ...
Fengxiang Zhao, Ruiqi Zhang, Rujun Wei, Huimin Fan, Yongfei Hu, Wenyu Shi, Jinfeng Wang   +6 more
wiley   +1 more source

Enhanced Antitumor Effect in Liver Cancer by Amino Acid Depletion-Induced Oxidative Stress

Frontiers in Oncology, 2021
Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer. HCC cells consume large amounts of glutamine to survive, but can adapt to glutamine depletion in the presence of an exogenous asparagine.
Keiichiro Okuda, Atsushi Umemura, Seita Kataoka, Kota Yano, Aya Takahashi, Shinya Okishio, Hiroyoshi Taketani, Yuya Seko, Taichiro Nishikawa, Kanji Yamaguchi, Michihisa Moriguchi, Hayato Nakagawa, Yu Liu, Yasuhide Mitsumoto, Yoshihiro Kanbara, Toshihide Shima, Takeshi Okanoue, Yoshito Itoh   +17 more
doaj   +1 more source

Ectopic Overexpression of Asparagine Synthetase in Transgenic Tobacco [PDF]

Plant Physiology, 1993
Here, we monitor the effects of ectopic overexpression of genes for pea asparagine synthetase (AS1) in transgenic tobacco (Nicotiana tabacum). The AS genes of pea and tobacco are normally expressed only during the dark phase of the diurnal growth cycle and specifically in phloem cells.
T., Brears, C., Liu, T. J., Knight, G. M., Coruzzi   +3 more
openaire   +2 more sources

Systematic Metabolic Engineering and Model‐Guided Optimization for High‐Level Production of L‐Theanine from Xylose in Escherichia coli

Advanced Science, EarlyView.
This study is pioneering in constructing the shortest known synthetic pathway for L‐theanine production from xylose within E coli. Through comprehensive metabolic engineering strategies, our engineered strain achieved the highest reported L‐theanine titer from xylose, with a titer of 95.42 g/L, and a yield of 0.55 g/g.
Haolin Han, Boyuan Xue, Guangqi Shan, Meng Meng, Shaojie Wang, Haijia Su   +5 more
wiley   +1 more source

Novel insights into regulation of asparagine synthetase in conifers

Frontiers in Plant Science, 2012
Asparagine, a key amino acid for nitrogen storage and transport in plants, is synthesized via the ATP-dependent reaction catalyzed by the enzyme asparagine synthetase (AS; EC 6.3.5.4).
Javier eCanales, Marina eRueda-López, Blanca eCraven-Bartle, Concepción eAvila, Francisco M Cánovas   +4 more
doaj   +1 more source