Results 81 to 90 of about 11,688 (172)

Crystal structure of plant γ‐glutamyl peptidase 1: implications for sulfur metabolism and secondary metabolite biosynthesis

The FEBS Journal, Volume 293, Issue 5, Page 1400-1414, March 2026.
The crystal structures of Arabidopsis thaliana γ‐glutamyl peptidase 1 (GGP1), including the covalent intermediate state, were determined. GGP1 is involved in both glutathione degradation in the primary metabolism and glutathione conjugate processing in the secondary metabolism of glucosinolate and camalexin biosynthesis.
Kosei Sone, Takehiro Ito, Hibiki Sawada, Chihaya Yamada, Toma Kashima, Akimasa Miyanaga, Naoko Ohkama‐Ohtsu, Shinya Fushinobu   +7 more
wiley   +1 more source

B‐GATA factors are required for nitrogen‐responsive growth in Physcomitrium patens and Arabidopsis thaliana

New Phytologist, Volume 249, Issue 6, Page 2919-2937, March 2026.
Summary We hypothesized that B‐GATA family transcription factors have important roles in growth regulation in moss. We analyzed B‐GATA family transcription factor mutants from Physcomitrium patens and Arabidopsis thaliana to assess growth, gene expression, and cytokinin‐related processes under varying nitrogen conditions.
Dario Zappone, Peter Michael Schröder, Ivan Petřík, Xiao Dong, Rudi Schäufele, Korbinian Schneeberger, Ondřej Novák, Claus Schwechheimer   +7 more
wiley   +1 more source

Clinical whole exome sequencing from dried blood spot identifies novel genetic defect underlying asparagine synthetase deficiency

Clinical Case Reports, 2018
Key Clinical Message We add two novel variants to the existing mutation spectrum of ASNS gene. Loss of ASNS function should be suspected in newborns presenting with congenital microcephaly, intellectual disability, progressive cerebral atrophy, and ...
Avinash Abhyankar, Michelle Lamendola‐Essel, Kelly Brennan, Jessica L. Giordano, Cecilia Esteves, Vanessa Felice, Ronald Wapner, Vaidehi Jobanputra   +7 more
doaj   +1 more source

Expression of Human Asparagine Synthetase in Escherichia Coli

Journal of Biological Chemistry, 1989
Human asparagine synthetase was expressed in Escherichia coli. Synthesis of the enzyme was demonstrated by immunoblotting and by complementation of asparagine auxotrophy in E. coli. The recombinant enzyme was shown to have both the ammonia- and glutamine-dependent asparagine synthetase activity in vitro.
G, Van Heeke, S M, Schuster
openaire   +2 more sources

Optimization of carbon and nitrogen utilization for enhanced photosynthesis, biomass and yield by coexpression of phosphoenolpyruvate carboxylase, aspartate aminotransferase and glutamine synthetase in Brassica juncea

Plant Biotechnology Journal, Volume 24, Issue 3, Page 1110-1132, March 2026.
Summary Coexpression of genes involved in carbon (C) and nitrogen (N) metabolism offers a promising avenue for improving crop yield. This study investigated the impact of coexpressing phosphoenolpyruvate carboxylase [ZmPEPC (P)], aspartate aminotransferase [GmAspAT (A)] and glutamine synthetase [NtGS (G)] in Brassica juncea to enhance plant yield ...
Mamta, Shiv Shanker Pandey, Anish Kaachra, Sachin Vashisath, Dinesh Kumar, Sanjay Kumar   +5 more
wiley   +1 more source

Asparagine Availability Is a Critical Limiting Factor for Infectious Spleen and Kidney Necrosis Virus Replication

Viruses
Infectious spleen and kidney necrosis virus (ISKNV) has brought huge economic loss to the aquaculture industry. Through interfering with the viral replication and proliferation process that depends on host cells, its pathogenicity can be effectively ...
Baofu Ma, Fangying Li, Xiaozhe Fu, Xia Luo, Qiang Lin, Hongru Liang, Yinjie Niu, Ningqiu Li   +7 more
doaj   +1 more source

Asparagine Synthetase Deficiency causes reduced proliferation of cells under conditions of limited asparagine

Molecular Genetics and Metabolism, 2015
Asparagine Synthetase Deficiency is a recently described cause of profound intellectual disability, marked progressive cerebral atrophy and variable seizure disorder. To date there has been limited functional data explaining the underlying pathophysiology. We report a new case with compound heterozygous mutations in the ASNS gene (NM_183356.3:c.
Palmer, EE, Hayner, J, Sachdev, R, Cardamone, M, Kandula, T, Morris, P, Dias, KR, Tao, J, Miller, D, Zhu, Y, Macintosh, R, Dinger, ME, Cowley, MJ, Buckley, MF, Roscioli, T, Bye, A, Kilberg, MS, Kirk, EP, Palmer, Elizabeth   +18 more
openaire   +3 more sources

Reaction hijacking inhibition of Plasmodium falciparum asparagine tRNA synthetase

Nature Communications
Malaria poses an enormous threat to human health. With ever increasing resistance to currently deployed drugs, breakthrough compounds with novel mechanisms of action are urgently needed.
Stanley C. Xie, Yinuo Wang, Craig J. Morton, Riley D. Metcalfe, Con Dogovski, Charisse Flerida A. Pasaje, Elyse Dunn, Madeline R. Luth, Krittikorn Kumpornsin, Eva S. Istvan, Joon Sung Park, Kate J. Fairhurst, Nutpakal Ketprasit, Tomas Yeo, Okan Yildirim, Mathamsanqa N. Bhebhe, Dana M. Klug, Peter J. Rutledge, Luiz C. Godoy, Sumanta Dey, Mariana Laureano De Souza, Jair L. Siqueira-Neto, Yawei Du, Tanya Puhalovich, Mona Amini, Gerry Shami, Duangkamon Loesbanluechai, Shuai Nie, Nicholas Williamson, Gouranga P. Jana, Bikash C. Maity, Patrick Thomson, Thomas Foley, Derek S. Tan, Jacquin C. Niles, Byung Woo Han, Daniel E. Goldberg, Jeremy Burrows, David A. Fidock, Marcus C. S. Lee, Elizabeth A. Winzeler, Michael D. W. Griffin, Matthew H. Todd, Leann Tilley   +43 more
doaj   +1 more source

Differential effect of asparagine and glutamine removal on three adenocarcinoma cell lines

Heliyon
Asparagine and glutamine depletion operated by the drug Asparaginase (ASNase) has revolutionized therapy in pediatric patients affected by Acute Lymphoblastic Leukemia (ALL), bringing remissions to a remarkable 90 % of cases.
Greta Pessino, Leonardo Lonati, Claudia Scotti, Silvia Calandra, Ornella Cazzalini, Ombretta Iaria, Andrea Previtali, Giorgio Baiocco, Paola Perucca, Anna Tricarico, Martina Vetro, Lucia Anna Stivala, Carlo Ganini, Marta Cancelliere, Massimo Zucchetti, Isabella Guardamagna, Maristella Maggi   +16 more
doaj   +1 more source

Analysis of Enzyme Activity and Cellular Function for the N80S and S480F Asparagine Synthetase Variants Expressed in a Child with Asparagine Synthetase Deficiency. [PDF]

Int J Mol Sci, 2022
Staklinski SJ, Snanoudj S, Guerrot AM, Vanhulle C, Lecoquierre F, Bekri S, Kilberg MS.   +6 more
europepmc   +1 more source