Results 111 to 120 of about 4,614,031 (312)

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

PICALM::MLLT10 translocated leukemia

FEBS Letters, EarlyView.
This comprehensive review of PICALM::MLLT10 translocated acute leukemia provides an in‐depth review of the structure and function of CALM, AF10, and the fusion oncoprotein (1). The multifaceted molecular mechanisms of oncogenesis, including nucleocytoplasmic shuttling (2), epigenetic modifications (3), and disruption of endocytosis (4), are then ...
John M. Cullen, Antonia C. Nakatsugawa, Natalie Barton, Henry Haines, Gary S. Stein, Janet L. Stein, Daniel S. Wechsler, Jessica L. Heath   +7 more
wiley   +1 more source

More and better information to tackle HIV epidemics: towards improved HIV incidence assays. [PDF]

PLoS Medicine, 2011
Incidence Assay Critical Path Working Group
doaj   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Enzyme-linked immunosorbent assay for urinary albumin at low concentrations [PDF]

, 1987
We describe an enzyme-linked immunosorbent assay (ELISA) for urinary albumin. It requires only commercially available reagents, can detect as little as 16 micrograms of albumin per liter, and analytical recovery ranges from 92 to 116%.
Jesse, U., Krämer, Bernhard K., Ress, K. M., Risler, T., Schmülling, R. M.   +4 more
core  

Contamination Control and Assay Results for the Majorana Demonstrator Ultra Clean Components

, 2017
The MAJORANA DEMONSTRATOR is a neutrinoless double beta decay experiment utilizing enriched Ge-76 detectors in 2 separate modules inside of a common solid shield at the Sanford Underground Research Facility.
Abgrall, N., Alvis, S. I., Arnquist, I. J., Avignone III, F. T., Barabash, A. S., Barton, C. J., Bertrand, F. E., Bode, T., Bradley, A. W., Brudanin, V., Busch, M., Buuck, M., Caldwell, T. S., Chan, Y-D., Christofferson, C. D., Chu, P. -H., Cuesta, C., Detwiler, J. A., Dunagan, C., Efremenko, Yu., Ejiri, H., Elliott, S. R., Gilliss, T., Giovanetti, G. K., Green, M. P., Gruszko, J., Guinn, I. S., Guiseppe, V. E., Haufe, C. R., Hehn, L., Henning, R., Hoppe, E. W., Howe, M. A., Keeter, K. J., Kidd, M. F., Konovalov, S. I., Kouzes, R. T., Lopez, A. M., Martin, R. D., Massarczyk, R., Meijer, S. J., Mertens, S., Myslik, J., O'Shaughnessy, C., Othman, G., Poon, A. W. P., Radford, D. C., Rager, J., Reine, A. L., Rielage, K., Robertson, R. G. H., Rouf, N. W., Shanks, B., Shirchenko, M., Suriano, A. M., Tedeschi, D., Trimble, J. E., Varner, R. L., Vasilyev, S., Vetter, K., Vorren, K., White, B. R., Wilkerson, J. F., Wiseman, C., Xu, W., Yakushev, E., Yu, C. -H., Yumatov, V., Zhitnikov, I., Zhu, B. X.   +69 more
core   +1 more source

Immuno-Assays

, 2022
Immuno-assays are helpful serological techniques for the laboratory diagnosis of autoimmune blistering diseases (AIBD) and for monitoring disease activity of individual patients. The three main immuno-assays are immunoblotting, immunoprecipitation and ELISA.
openaire   +2 more sources

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source