Results 121 to 130 of about 312,451 (313)

USP7 inhibits FOXO1 activity in chronic lymphocytic leukaemia cells enhancing cell survival

Molecular Oncology, EarlyView.
CLL cells rely on BCR‐mediated signalling for survival and proliferation in part through suppression of FOXO1. FOXO1 activity is regulated by a plethora of post‐translational modifications including phosphorylation and ubiquitination. We demonstrated that dual inhibition of BCR signalling and the deubiquitinase USP7 activates FOXO1, modulating its ...
Hassan N.B. Almuhanna, Jodie Hay, Michael W. Moles, Lucy Clarke, Jennifer Cassels, Alison Sefcick, Alison M. McCaig, Heather G. Jørgensen, Alison M. Michie   +8 more
wiley   +1 more source

Tumour‐proximal deep serum proteomics identifies biomarkers associated with ovarian cancer

Molecular Oncology, EarlyView.
New biomarkers are needed for early ovarian cancer detection. Ovarian (tumour‐proximal) and peripheral vein serum from healthy women and patients with benign or malignant tumours underwent nanoparticle‐enriched deep proteomics. Ovarian serum yielded more protein identifications and revealed tumour‐reflective signatures.
Darren Lau, Daniel Pincher, Clifford Young, Nicholas Chappell, Noor A. Lokman, Parul Mittal, Leigh Donnellan, Martin K. Oehler, Doug A. Brooks, Peter Hoffmann   +9 more
wiley   +1 more source

Barlow Twins deep neural network for advanced 1D drug–target interaction prediction

Journal of Cheminformatics
Accurate prediction of drug–target interactions is critical for advancing drug discovery. By reducing time and cost, machine learning and deep learning can accelerate this laborious discovery process.
Maximilian G. Schuh, Davide Boldini, Annkathrin I. Bohne, Stephan A. Sieber   +3 more
doaj   +1 more source

In Silico Analysis of Potential Stabilizer Binding Sites at Protein–RNA Interfaces

Computational and Structural Biotechnology Journal
Highlights • First large-scale in silico investigation of molecular glue druggability in protein–RNA complexes, combining virtual screening, molecular dynamics simulations, and structure prediction models.• A total of 52,000,000 ligand–receptor complexes
Luis Vollmers, Shu-Yu Chen, Martin Zacharias   +2 more
doaj   +1 more source

Morphological Transformations of Nonequilibrium Assemblies of Amphiphilic Diblock Copolymer

, 2014
Amphiphilic diblock copolymer polystyrene-block-poly(ethylene oxide) (PS-PEO) assembled into nonequilibrium bicontinuous structures or mixture of vesicles, bilayers and nanorods upon rapid micellization induced by rapid addition of selective solvent ...
Zhou, Qi, Cong, Yang, Fu, Jun, Fang, Jianghua, Chen, Bin   +4 more
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

On Candidate-Based Analyses of Assembly Elections. [PDF]

Analyses of assembly elections often assume that voters have well-defined preferences over candidates, even though preferences over assemblies are the natural analytic starting point.
Kornhauser, L.A., Benoit, J.P.
core  

Taxonomic classification of assemblies.

, 2013
Assemblies were classified based on comparison to the CAMERA database using the BLASTX algorithm and an e-value of 1×10e-5 or lower. Sequences in assemblies without significant matches to existing protein sequences (e-value>1E-5) were classified as ...
David J. Spiro (52457), Ryan Kuzmickas (41917), Appolinaire Djikeng (41915), Norman G. Anderson (370218)   +3 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

How conformational changes near the F pocket of MHC class I proteins mediate chaperone assisted peptide loading

Frontiers in Immunology
BackgroundEfficient recognition of antigenic peptides bound to major histocompatibility complex (MHC) class I molecules on the surface of cells by immune cells requires sufficiently stable peptide-MHC I complexes.
Simone Göppert, Sebastian Springer, Martin Zacharias   +2 more
doaj   +1 more source