Results 101 to 110 of about 176,761 (289)
ATAC-seq in Xenopus laevis gastrula stage
, 2019 The hypothesis being tested is that loss of competence to respond to inductive signals is mediated by loss of chromatin accessibility. This dataset describes analysis of an Assay for Transposase-Accessible Chromatin followed by sequencing (ATAC-Seq ...
Kai Xhang (7510208) +5 more
core +2 more sources
ATAC-seq analysis in human CD4+ T-cell subsets.
, 2022 (A) Memory T cells were sorted from peripheral blood and were either left resting or activated with plate-bound anti-CD3 and anti-CD28 antibodies for the indicated number of hours. Total RNA was extracted and miR-150 expression was measured by RT-qPCR. N
Michele Chirichella (4025858) +7 more
core +1 more source
Establishing an optimized ATAC-seq protocol for the maize
Frontiers in Plant ScienceThe advent of next-generation sequencing in crop improvement offers unprecedented insights into the chromatin landscape closely linked to gene activity governing key traits in plant development and adaptation. Particularly in maize, its dynamic chromatin structure is found to collaborate with massive transcriptional variations across tissues and ...
Jo-Wei Allison Hsieh +10 more
openaire +3 more sources
Advanced Science, EarlyView.HIV preferentially infects microglia, activating IL‐17A‐driven IFN‐γ/STAT1, CEBPB, and NF‐κB signaling that converges with cGAS‐STING and Notch–NEURL1 pathways, promoting neuroinflammation, mitochondrial dysfunction, and synaptic impairment. cART suppresses viral replication, attenuates inflammatory signaling, and at least partially restores ...
Karthick Chennakesavan +7 more
wiley +1 more source
A rapid and robust method for single cell chromatin accessibility profiling
Nature Communications, 2018 ATAC-seq is widely used to identify regulatory regions in the genome. Here the authors develop a simple and robust plate-based single-cell ATAC-seq method that works in fresh and cryopreserved cells.
Xi Chen +3 more
doaj +1 more source
ATAC-seq on dissected, frozen, embryo halves.
, 2018 (A) Stage 5, hand sorted Drosophila embryos were flash frozen over dry ice in a buffer containing 5% glycerol and manually sliced in half with a scalpel. Twenty anterior and posterior halves were collected, homogenized, and the nuclei were isolated. ATAC-
Michael B. Eisen (13535) +1 more
core +1 more source
Advanced Science, EarlyView.DUET‐seq is an open‐source droplet platform that jointly profiles chromatin accessibility and gene expression from the same nucleus. Dissolvable dual‐linker hydrogel beads and a one‐step intra‐droplet RT‐PCR co‐index both modalities in under 12 h at roughly $0.04 per cell.
Dong Cheng +16 more
wiley +1 more source
MutiQC Report for Neuroblastoma Cell Line ATAC-Seq and ChIP-Seq
, 2020 MutiQC Report for Neuroblastoma Cell Line ATAC-Seq and ChIP ...
Apexa Modi (8526572) +1 more
core +1 more source
A simple ATAC-seq protocol for population epigenetics
Wellcome Open Research, 2020 We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions.
Ronaldo de Carvalho Augusto +2 more
openaire +1 more source