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Atg8: an autophagy-related ubiquitin-like protein family [PDF]

Genome Biology, 2011
Autophagy-related (Atg) proteins are eukaryotic factors participating in various stages of the autophagic process. Thus far 34 Atgs have been identified in yeast, including the key autophagic protein Atg8. The Atg8 gene family encodes ubiquitin-like proteins that share a similar structure consisting of two amino-terminal α helices and a ubiquitin-like ...
Tomer Shpilka, Hilla Weidberg, Shmuel Pietrokovski   +2 more
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Membrane perturbation by lipidated Atg8 underlies autophagosome biogenesis

Nature Structural & Molecular Biology, 2021
Autophagosome biogenesis is an essential feature of autophagy. Lipidation of Atg8 plays a critical role in this process. Previous in vitro studies identified membrane tethering and hemi-fusion/fusion activities of Atg8, yet definitive roles in autophagosome biogenesis remained controversial.
Tatsuro Maruyama, Jahangir Md. Alam, Tomoyuki Fukuda, Shun Kageyama, Hiromi Kirisako, Yuki Ishii, Ichio Shimada, Yoshinori Ohsumi, Masaaki Komatsu, Tomotake Kanki, Hitoshi Nakatogawa, Nobuo N. Noda   +11 more
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CHARACTERIZATION OF ATG8 IN LEPIDOPTERAN INSECT CELLS

Archives of Insect Biochemistry and Physiology, 2013
Yeast Atg8 and mammalian microtubule‐associated protein light chain 3 (LC3) are landmark proteins essential for autophagy. Here the lepidopteran Atg8, a homolog of LC3, is characterized. Sequence analysis reveals that Atg8 proteins are highly conserved in lepidopteran species.
Zhongchao, Gai, Xiaojuan, Zhang, Mayira, Islam, Xia, Wang, Aiying, Li, Yongbo, Yang, Yi, Li, Jianxin, Peng, Huazhu, Hong, Kaiyu, Liu   +9 more
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Non-canonical roles of ATG8 for TFEB activation

Biochemical Society Transactions, 2022
Autophagy is an evolutionally conserved cytoplasmic degradation pathway in which the double membrane structure, autophagosome sequesters cytoplasmic material and delivers them to lysosomes for degradation. Many autophagy related (ATG) proteins participate in the regulation of the several steps of autophagic process.
Shuhei Nakamura, Shiori Akayama, Tamotsu Yoshimori   +2 more
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New tricks for the old autophagy protein Atg8

Nature Structural & Molecular Biology, 2021
The autophagy protein Atg8 regulates autophagosome biogenesis and mediates cargo recruitment in different types of selective autophagy, but the molecular mechanisms mediating Atg8 functions during autophagosome biogenesis have been unclear.
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Studies of Receptor-Atg8 Interactions During Selective Autophagy

2019
Autophagy research frequently requires the determination of protein-protein interactions. The experimental system described in this chapter allows a simple, versatile, and quantitative in vitro analysis of interactions between recombinant cargo receptor and Atg8 proteins by fluorescence microscopy.
Abert, Christine, Martens, Sascha
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Characterization of ATG8-Family Interactors by Isothermal Titration Calorimetry

2022
Isothermal titration calorimetry (ITC) is the gold standard for providing quantitative and thermodynamic understanding of the interaction mechanisms between core autophagy machinery, autophagy receptors, and ATG8. Here, we used two model peptides and Arabidopsis thaliana ATG8A to characterize ATG8-peptide interactions.
Lorenzo, Picchianti, Arthur, Sedivy, Yasin, Dagdas   +2 more
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In Vitro Reconstitution of Atg8 Conjugation and Deconjugation

2017
Macroautophagy, hereafter autophagy, is a major degradation pathway in eukaryotic systems that allows the removal of large intracellular structures such as entire organelles or protein aggregates, thus contributing to the homeostasis of cells and tissues.
Fracchiolla, Dorotea, Zens, Bettina, Martens, Sascha   +2 more
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2020
Morphological and cytological analyses of Arabidopsis thaliana seedlings roots growing under conditions mimicking a microgravity has been performed. Moreover, we assessed the process of autophagy induction in cells upon such conditions. Here, we report significant changes in the root development and the enhanced autophagosome formation in epidermal ...
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Investigating Structure and Dynamics of Atg8 Family Proteins

2017
Atg8 family members were the first autophagy-related proteins to be investigated in structural detail and continue to be among the best-understood molecules of the pathway. In this review, we will first provide a concise outline of the major methods that are being applied for structural characterization of these proteins and the complexes they are ...
O H, Weiergräber, M, Schwarten, B, Strodel, D, Willbold   +3 more
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