Results 61 to 70 of about 44,944 (293)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Axially Ligated Mesohemins as Bio-Mimicking Catalysts for Atom Transfer Radical Polymerization

Molecules, 2019
Copper is the most common metal catalyst used in atom transfer radical polymerization (ATRP), but iron is an excellent alternative due to its natural abundance and low toxicity compared to copper.
Liye Fu, Antonina Simakova, Sangwoo Park, Yi Wang, Marco Fantin, Krzysztof Matyjaszewski   +5 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Orthogonal surface functionalization through bioactive vapor‐based polymer coatings [PDF]

, 2014
Reactive chemical vapor deposition (CVD) polymerization provides a substrate‐independent platform for effective functionalization of virtually any solid substrates, flat, or curved, even with complex geometries.
Alf, Almeida, Azioune, Broyer, Campos, Chang, Chen, Chen, Chen, Christman, Collioud, del Campo, Demers, Deng, Deng, Deng, Desmet, Edmondson, Elkasabi, Elkasabi, Elkasabi, Elkasabi, Grabarek, Gromada, Han, Hermanson, Hoa, Hoyle, Hui, Im, Im, Inglis, Jakubowski, Jiang, Kade, Kalia, Kalia, Katz, Kolb, Kozai, Lahann, Lahann, Lahann, Lahann, Lallana, Lesaicherre, Li, Magenau, Mari-Buye, Mattson, Matyjaszewski, Matyjaszewski, Matyjaszewski, Matyjaszewski, Meldal, Meyer-Plath, Min, Montero, Nandivada, Nandivada, Nebhani, Ning, O'Shaughnessy, Pirrung, Prucker, Ratner, Ross, Rostovtsev, Siegwart, Simon, Sletten, Slowing, Strother, Szwarc, Tawfick, Tsai, Turkova, Ulman, Wang, Wu, Yan, Zhang   +81 more
core   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Synthesis and Characterization of Benzodioxinone-Bearing Methacrylate-Based Random Copolymer via Atom Transfer Radical Polymerization

Journal of the Turkish Chemical Society, Section A: Chemistry, 2021
Methacrylate-benzodioxinone (BDMA) as a monomer was firstly synthesized and then copolymerized with methyl methacrylate and 2-hydroxyethyl methacrylate via atom transfer radical polymerization (ATRP) in the presence of CuCl/pentamethyldiethylene triamine
Cumali ÇELİK, Gokhan ACİK
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Synthesis and reactivity of self-immolative pDTT-g-pGMA bottlebrush polymer

eXPRESS Polymer Letters
Post-polymerization modification of self-immolative polymers expands the application spectrum of such macromolecular materials. This work demonstrates grafting of poly(glycidyl methacrylate) brushes from a self-immolative poly(DL-dithiothreitol) backbone
Marie Uth, Magnus Hansen-Felby, Jakob Rørbæk Saugbjerg, Andreas Sommerfeldt, Sunirmal Pal, Martin Lahn Henriksen, Steen Uttrup Pedersen, Kim Daasbjerg   +7 more
doaj   +1 more source

Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

BMC Chemistry, 2019
A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction.
Lu Qi, Aihong Zhang, Yu Wang, Long Liu, Xinghe Wang   +4 more
doaj   +1 more source