Results 51 to 60 of about 613,647 (384)

Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy

Nature Communications, 2017
The CRISPR-associated endonuclease Cas9 binds to a guide RNA and cleaves double-stranded DNA with a sequence complementary to the RNA guide. The Cas9–RNA system has been harnessed for numerous applications, such as genome editing.
Mikihiro Shibata, H. Nishimasu, N. Kodera, S. Hirano, T. Ando, T. Uchihashi, O. Nureki   +6 more
semanticscholar   +1 more source

The cytoskeletal control of B cell receptor and integrin signaling in normal B cells and chronic lymphocytic leukemia

FEBS Letters, EarlyView.
In lymphoid organs, antigen recognition and B cell receptor signaling rely on integrins and the cytoskeleton. Integrins act as mechanoreceptors, couple B cell receptor activation to cytoskeletal remodeling, and support immune synapse formation as well as antigen extraction.
Abhishek Pethe, Tanja Nicole Hartmann
wiley   +1 more source

Investigating Atomic Details of the CaF$_2$(111) Surface with a qPlus Sensor [PDF]

, 2004
The (111) surface of CaF$_2$ has been intensively studied with large-amplitude frequency-modulation atomic force microscopy and atomic contrast formation is now well understood.
Allers W, Barth C, Foster A, Franz J Giessibl, Giessibl F J, Giessibl F J, Giessibl F J, Giessibl F J, Giessibl F J, Israelachvili J, Letz M, Michael Reichling, Reichling M, Schick M, Tasker P W   +14 more
core   +2 more sources

Advances in atomic force microscopy for single-cell analysis

Nano Reseach, 2018
Single-cell analysis has been considered as a promising way to uncover the underlying mechanisms guiding the mysteries of life activities, which considerably complements traditional ensemble assays and yields novel insights into cell biology.
Mi Li, N. Xi, Yuechao Wang, Lianqing Liu
semanticscholar   +1 more source

Quantification of surface displacements and electromechanical phenomena via dynamic atomic force microscopy [PDF]

Nanotechnology, 2016
Detection of dynamic surface displacements associated with local changes in material strain provides access to a number of phenomena and material properties.
N. Balke, S. Jesse, P. Yu, Ben Carmichael, Sergei V. Kalinin, A. Tselev   +5 more
semanticscholar   +1 more source

Interaction vesicles as emerging mediators of host‐pathogen molecular crosstalk and their implications for infection dynamics

FEBS Letters, EarlyView.
Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke, Izadora Volpato Rossi, Marcel I. Ramirez   +2 more
wiley   +1 more source

Pectin Nanostructure Visualization by Atomic Force Microscopy

Bio-Protocol, 2015
Pectins, complex polysaccharides rich in galacturonic acid, are a major component of primary plant cell walls. These macromolecules regulate cell wall porosity and intercellular adhesion, being important in the control of cell expansion and ...
Sara Posé, Candelas Paniagua, Andrew Kirby, A. Gunning, Victor Morris, Miguel Quesada, Jose Mercado   +6 more
doaj   +1 more source

Structure of self-assembled Mn atom chains on Si(001) [PDF]

, 2015
Mn has been found to self-assemble into atomic chains running perpendicular to the surface dimer reconstruction on Si(001). They differ from other atomic chains by a striking asymmetric appearance in filled state scanning tunneling microscopy (STM ...
Bowler, D., Kirkham, Ch. J., Köster, S. A., Longobardi, M., Renner, Ch., Villarreal, R.   +5 more
core   +3 more sources

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source

SNAREs: Could They be the Answer to an Energy Landscape Riddle in Exocytosis?

The Scientific World Journal, 2010
During exocytosis, chemical transmitters stored in secretory vesicles can be released upon fusion of these intracellular organelles to the plasma membrane. In this process, SNARE proteins that form a ternary core complex play a central role. This complex
Wei Liu, Vladimir Parpura
doaj   +1 more source