Results 71 to 80 of about 179,289 (254)

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Laboratory Studies on Absolute n-resolved Charge-exchange Cross Sections and Modeling X-Ray Emissions for Ne8+ Colliding with H2 and He

The Astrophysical Journal
Charge exchange is a predominant process in inelastic collisions in astronomical plasmas, influencing the charge state balance and leading to X-ray emissions when solar wind ions interact with neutral gas in astrophysical environments.
P. F. Ma, H. Yin, B. Ren, T. Meng, J. Wang, Y. Wu, X. Tan, Y. Zhang, Y. Qi, K. Yao, J. Xiao, Y. Zou, B. Tu, B. Wei   +13 more
doaj   +1 more source

Unveiling the ESIPT Luminescence Mechanism of 4′-N,N-Diethylamino-3-Hydroxyflavone in Ionic Liquid: A Computational Study

Molecules
Excited state intramolecular proton transfer (ESIPT) within molecules in solvents plays important roles in photo-chemistry and photo-biology. Herein, the influence of 1-ethyl-3-methyl-imidazolium bis (trifluoromethylsulfonyl) imide ([EMIm][NTf2]) and 1 ...
Jin Yang, Qi Li, Meilin Guo, Lu Yan, Lixia Zhu, Jing Zhao, Guangxiong Hu, Hang Yin, Ying Shi   +8 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

G0-PCC-FISH derived multi-parametric biodosimetry methodology for accidental high dose and partial body exposures

Scientific Reports
High dose radiation exposures are rare. However, medical management of such incidents is crucial due to mortality and tissue injury risks. Rapid radiation biodosimetry of high dose accidental exposures is highly challenging, considering that they usually
Usha Yadav, Nagesh N. Bhat, Utkarsha S. Mungse, Kapil B. Shirsath, Manish Joshi, Balvinder K. Sapra   +5 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Design and development of a semi-automated solid target assembly for the indigenous production of medical radioisotopes in the PETrace-800 cyclotron

EJNMMI Radiopharmacy and Chemistry
Background Molecular imaging enables the non-invasive visualization of physiological processes at the cellular and molecular levels and is central to diagnostic nuclear medicine. Among clinically important theranostic radionuclides, 64Cu and 111In are of
Kanchan Kushwaha, Kavindra Pathak, Tejinder P. Sabharwal, Sunil Kamble, Arpit Mitra, Sudeep Sahu, Pravind Maletha, Nand Kishore Prasad, Manish Kumar, Sharmila Banerjee   +9 more
doaj   +1 more source

Sensitivity to sterile neutrino mixing using reactor antineutrinos

European Physical Journal C: Particles and Fields, 2019
The reactor antineutrinos are used for the precise measurement of oscillation parameters in the 3-neutrino model, and also used to investigate active-sterile neutrino mixing sensitivity in the $$3+1$$ 3+1 neutrino framework. In the present work, we study
S. P. Behera, D. K. Mishra, L. M. Pant
doaj   +1 more source