A novel direct homogeneous assay for ATP citrate lyase[S] [PDF]
Journal of Lipid Research, 2009 ATP citrate lyase (ACL) is a cytosolic enzyme that catalyzes the synthesis of acetyl-CoA and oxaloacetate using citrate, CoA, and ATP as substrates and Mg2+ as a necessary cofactor.
Zhengping Ma +2 more
doaj +3 more sources
Impact of ATP-citrate lyase catalytic activity and serine 455 phosphorylation on histone acetylation and inflammatory responses in human monocytic THP-1 cells [PDF]
Frontiers in Immunology, 2022 ATP-citrate lyase (ACLY) is a key enzyme provoking metabolic and epigenetic gene regulation. Molecularly, these functions are exerted by the provision of acetyl-coenzyme A, which is then used as a substrate for de novo lipogenesis or as an acetyl-group ...
Monica Dominguez +7 more
doaj +2 more sources
ATP-citrate lyase B (ACLB) negatively affects cell death and resistance to Verticillium wilt [PDF]
BMC Plant Biology, 2022 Background ATP-citrate lyase (ACL) plays a pivotal role in histone acetylation and aerobic glycolysis. In plant, ACL is a heteromeric enzyme composed of ACLA (45 kD) and ACLB (65 kD).
Fujie Liu +5 more
doaj +2 more sources
Catalytic mechanism study of ATP-citrate lyase during citryl-CoA synthesis process [PDF]
iScienceSummary: ATP-citrate lyase (ACLY) is a critical metabolic enzyme and promising target for drug development. The structure determinations of ACLY have revealed its homotetramer states with various subunit symmetries, but catalytic mechanism of ACLY ...
Danfeng Shi +4 more
doaj +2 more sources
Myeloid ATP Citrate Lyase Regulates Macrophage Inflammatory Responses In Vitro Without Altering Inflammatory Disease Outcomes [PDF]
Frontiers in Immunology, 2021 Macrophages are highly plastic, key regulators of inflammation. Deregulation of macrophage activation can lead to excessive inflammation as seen in inflammatory disorders like atherosclerosis, obesity, multiple sclerosis and sepsis.
Sanne G. S. Verberk +14 more
doaj +2 more sources
ATP citrate lyase inhibition can suppress tumor cell growth [PDF]
Cancer Cell, 2005 Many tumors display a high rate of glucose utilization, as evidenced by 18-F-2-deoxyglucose PET imaging. One potential advantage of catabolizing glucose through glycolysis at a rate that exceeds bioenergetic need is that the growing cell can redirect the excess glycolytic end product pyruvate toward lipid synthesis.
Sunil R Hingorani +2 more
exaly +3 more sources
ATP Citrate Lyase Regulates Myofiber Differentiation and Increases Regeneration by Altering Histone Acetylation [PDF]
Cell Reports, 2017 ATP citrate lyase (ACL) plays a key role in regulating mitochondrial function, as well as glucose and lipid metabolism in skeletal muscle. We report here that ACL silencing impairs myoblast and satellite cell (SC) differentiation, and it is accompanied ...
Suman Das +10 more
doaj +2 more sources
mTORC2-AKT signaling to ATP-citrate lyase drives brown adipogenesis and de novo lipogenesis
Nature Communications, 2020 mTORC2 activates Akt, a regulator of cell growth and metabolism, however, the role of mTORC2 in adipocytes is incompletely understood. Here the authors report that a mTORC2-Akt axis specifically activates ACLY to promote lipid synthesis and histone ...
Amelia Luciano +2 more
exaly +2 more sources
Nature Communications, 2016 Statins are lipid-lowering drugs that prevent cardiovascular disease but tolerability is limited by severe side effects in muscles. Here the authors elucidate a liver-specific activation mechanism for bempedoic acid, a novel cholesterol-lowering drug ...
Emily A Day +2 more
exaly +2 more sources
Molecular basis for acetyl-CoA production by ATP-citrate lyase. [PDF]
Nat Struct Mol Biol, 2020 ATP‐citrate lyase (ACLY) synthesizes cytosolic acetyl‐CoA, a fundamental cellular building block. Accordingly, aberrant ACLY activity is observed in many diseases. Here we report cryo‐EM structures of human ACLY alone or bound to substrates or products.
Wei X +4 more
europepmc +4 more sources