Results 251 to 260 of about 345,136 (302)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Mechanisms of ATP Release by Inflammatory Cells [PDF]

International Journal of Molecular Sciences, 2018
Extracellular nucleotides (e.g., ATP, ADP, UTP, UDP) released by inflammatory cells interact with specific purinergic P2 type receptors to modulate their recruitment and activation. The focus of this review is on stimuli and mechanisms of extracellular nucleotide release and its consequences during inflammation.
Michel Dosch, Fadi Jebbawi, Guido Beldi   +2 more
exaly   +6 more sources

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Purinergic Signalling: ATP Release

Neurochemical Research, 2001
Adenosine triphosphate (ATP) has a fundamental intracellular role as the universal source of energy for all living cells. The demonstration of its release into the extracellular space and the identification and localisation of specific receptors on target cells have been essential in establishing, after considerable resistance, its extracellular ...
P, Bodin, G, Burnstock
openaire   +2 more sources

ATP-Stimulated Release of ATP by Human Endothelial Cells

Journal of Cardiovascular Pharmacology, 1996
We investigated the effects of several concentrations of extracellular ATP on the release of intracellular ATP by human umbilical vein endothelial cells (HUVEC) in primary cultures. When ATP is added to the medium of cultured EC at a concentration of 1 microM, it is readily degraded by extracellular enzymes; 10 microM ATP added to the culture medium ...
P, Bodin, G, Burnstock
openaire   +2 more sources

Neural Release of ATP and Adenosine^a

Annals of the New York Academy of Sciences, 1990
Release of ATP can be evoked from noradrenergic nerve varicosities isolated from guinea pig ileal myenteric plexus by depolarization with K+ and veratridine and during exposure to acetylcholine or 5-HT. Clonidine, however, modulates the release of [3H]noradrenaline without affecting the release of ATP.
T D, White, W F, MacDonald
openaire   +2 more sources

Vesicular release of ATP at central synapses

Pflügers Archiv - European Journal of Physiology, 2006
Adenosine triphosphate (ATP) acts as a fast excitatory transmitter in several regions of the central nervous system (CNS) including the medial habenula, dorsal horn, locus coeruleus, hippocampus, and somatosensory cortex. Postsynaptic actions of ATP are mediated through an extended family of P2X receptors, widely expressed throughout the CNS.
Pankratov, Yuri, Lalo, Ulyana, Verkhratsky, Alexei; id_orcid 0000-0003-2592-9898, North, R. Alan   +3 more
openaire   +3 more sources

Retrograde Inhibition of Transmitter Release by ATP

Journal of Neurochemistry, 1980
Abstract: After labelling ACh tissue stores in Torpedo electric organ prisms with radioactive acetate, the release of ACh and ATP triggered by electrical stimulation or KCI depolarization was measured in the same perfusate samples. The luciferin‐luciferase reaction for ATP was first counted, then the radioactive content of the sample determined ...
M, Israël, B, Lesbats, R, Manaranche, F M, Meunier, P, Frachon   +4 more
openaire   +2 more sources