Biotechnology and Bioengineering, EarlyView.ABSTRACT The glutamine synthetase expression system is widely used for therapeutic protein biomanufacturing in Chinese hamster ovary (CHO) cells. Here, we present the design of an enhanced glutamine synthetase vector for generating CHOK1SV GS‐KO stable pools that produce recombinant proteins at multigram‐per‐liter levels. We first developed a synthetic
Yusuf B. Johari +11 more
wiley +1 more source
Metabolic imaging for gamete and embryo assessment through advanced microscopy technologies: a novel avenue for artificial intelligence? [PDF]
Hum ReprodHorta F +8 more
europepmc +1 more source
Bridging the translational gap in clinical nanomedicine: From rational design to clinical reality
BMEMat, EarlyView.Nanotechnology offers a multi‐faceted approach to modern healthcare, encompassing high‐sensitivity diagnostic imaging, innovative vaccine delivery, and targeted therapeutics. This review explores how these nanomedicine applications address critical challenges in cancer recurrence and the treatment of neurologic and respiratory diseases to combat rising
Ahmed H. Ghonaim +9 more
wiley +1 more source
Long-term natural history of spontaneous closure of a full-thickness macular hole in retinitis pigmentosa. [PDF]
BMJ Case RepRuan CW, Schneiders MG, Raja M.
europepmc +1 more source
RhodaMal Dyes: Novel Red Emissive Fluorophores as Probes for High Contrast Live‐Cell Imaging
Chemistry – A European Journal, EarlyView.Incorporation of malononitrile into the traditional rhodol framework furnishes RhodaMal (Rhoda = Rhodamine, Mal = Malononitrile;) dyes, a new class of red emissive push–pull chromophores. Conjugating them to triphenylphosphonium cation enables live cell super‐resolution STED microscopy with a 775 nm depletion laser.
A. Mavroskoufis +8 more
wiley +1 more source
Navigating imaging artifacts: challenges with visualizing intrinsically fluorescent antimalarials. [PDF]
Front ParasitolBremers EK +4 more
europepmc +1 more source
Chemistry – A European Journal, EarlyView.We report a cellular membrane‐targeted europium(III) probe, Eu.Chol, that enables selective, time‐resolved detection of peroxynitrite (ONOO−) at the cell membrane interface. Eu.Chol demonstrates efficient incorporation into liposomes and Jurkat cells, with minimal response to H2O2, establishing a modular platform for subcellular ONOO− sensing ...
Jamie Webb +6 more
wiley +1 more source
Photostability of Cyanine Dyes and Its Correlation to Their Photothermal and Photodynamic Effects
Chemistry – A European Journal, EarlyView.Photostability (PS) of dyes is a crucial parameter for performance in biological applications, yet often underrated. Here PS of several cyanines is investigated. The method is carefully evaluated and designed as a general and easy tool to compare PS of NIR dyes.
Clara Schäfer, Bo W. Laursen
wiley +1 more source
The Utility of PTeye in Parathyroidectomy for Recurrent Hyperparathyroidism Due to Intramuscular Parathyroid Autograft Hyperplasia: A Case Report. [PDF]
Sisli Etfal Hastan Tip BulHaciyanli M +7 more
europepmc +1 more source
Long Fluorescence Lifetime Molecular Rotors Based on the 4,4‐Dicyano‐BODIPY Core
Chemistry – A European Journal, EarlyView.Substituting fluorine with cyano groups in BODIPY‐based fluorescent molecular rotors yields viscosity‐sensitive dyes with significantly enhanced fluorescence lifetimes that can be used for viscosity mapping in living cells using FLIM microscopy. ABSTRACT Environmentally sensitive fluorescent probes with extended emission lifetimes are highly valuable ...
Yelyzaveta Denysieva +4 more
wiley +1 more source