Results 21 to 30 of about 81,928 (262)

Subcellular Localization and Vesicular Structures of Anthocyanin Pigmentation by Fluorescence Imaging of Black Rice (Oryza sativa L.) Stigma Protoplast

Plants, 2021
Anthocyanins belong to the group of flavonoid compounds broadly distributed in plant species responsible for attractive colors. In black rice (Oryza sativa L.), they are present in the stems, leaves, stigmas, and caryopsis.
Enerand Mackon, Yafei Ma, Guibeline Charlie Jeazet Dongho Epse Mackon, Qiufeng Li, Qiong Zhou, Piqing Liu   +5 more
doaj   +1 more source

Bacterial metabolites influence the autofluorescence of Clostridioides difficile

Frontiers in Microbiology
Clostridioides difficile is a bacterial pathogen that has been implicated in severe gastrointestinal infections. C. difficile has intrinsic green autofluorescence and the level of this autofluorescence is known to be increased by growth time and oxygen ...
Taylor D. Ticer, Anna M. Tingler, Janiece S. Glover, Sarah A. Dooley, Jacob Kendrick, Joseph P. Zackular, Joseph P. Zackular, Suzanne Devkota, Gary D. Wu, Karley Mahalak, Amy Engevik, Melinda A. Engevik, Melinda A. Engevik   +12 more
doaj   +1 more source

Biological mechanisms underlying autofluorescence loss in oral potentially malignant disorders: implications for clinical interpretation

Photodiagnosis and Photodynamic Therapy
Objective: To review the biological mechanisms underlying autofluorescence loss in oral potentially malignant disorders and their implications for clinical interpretation of oral mucosal autofluorescence findings.
Xiangyu Li, Eun-Song Lee, Baek-Il Kim, Hyung Jun Kim   +3 more
doaj   +1 more source

Decreased green autofluorescence in cancerous tissues is a potential biomarker for diagnosis of renal cell carcinomas

Scientific Reports
This study primarily focuses on the potential sources of autofluorescence, including keratins (KRT) encoded by KRT1, KRT7, and KRT8, to investigate their contributions to the differences in autofluorescence between cancerous tissues and adjacent non ...
Wei Wan, Junrong Zou, Tianpeng Xie, Liang Zeng, Huiquan Liu, Bo Jiang, Yunfeng Liao, Yuting Wu, Gengqing Wu, Guoxi Zhang, Weihai Ying, Xiaofeng Zou   +11 more
doaj   +1 more source

Pharmacological chromatin remodeling enhances response to estrogen therapy in ER+ breast cancer

Molecular Oncology, EarlyView.
Estrogen therapy elicits clinical benefit in ~ 30% of patients with endocrine‐resistant estrogen receptor (ER)‐positive breast cancer. Based on findings that ER transcriptional activation underlies response to estrogen therapy, we tested the effects of epigenetic dysregulation via pharmacological inhibition of histone deacetylases (HDACi).
Anneka L. Johnson Thomas, Barbara Karakyriakou, Patricia C. Muskus, Alyssa M. Roberts, Justin Rendleman, Alexa Warren, Hanxu Lu, Kimberly A. Birmingham, Malone K. Friedman, Abigail E. Goen, Huijuan Yang, Owen M. Wilkins, Fred W. Kolling, Eugene Demidenko, Julie M. Jorns, Brock C. Christensen, Todd W. Miller   +16 more
wiley   +1 more source

Bridging the gap: a genetically validated avian chorioallantoic membrane platform for investigation of spontaneous circulating tumor cells

Molecular Oncology, EarlyView.
We established the avian chorioallantoic membrane (CAM) assay as a scalable in vivo model for studying circulating tumor cells (CTCs). Human gastrointestinal tumors spontaneously released genetically validated CTCs that were detected across multiple platforms, demonstrating that the CAM model provides an accessible tool for investigating early cancer ...
Dennis Roth, Rui P. L. Neves, Julia Geheb, Christian Ballmeyer, Nathalie Gaßmann, Kiki C. Andree, Monica Sudarsanam, Christiane Driemel, Celine Örencik, Andrè Franken, Eslam Elmaghraby, Sven H. Loosen, Roderburg Christoph, Sebastian Schoelch, Hans Neubauer, Wolfram T. Knoefel, Nikolas H. Stoecklein, Georg Fluegen   +17 more
wiley   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Autofluorescence of Pathogenic Fungi

The Tohoku Journal of Experimental Medicine, 1964
Autofluorescence of pathogenic fungi was investigated. Smears were made from the surface of the growth on 4% glucose agar and were fixed by heating, absolute alcohol or formalin, and were placed under the fluorescence microscope. The fungi tested showed various kinds of autofluorescence.
Miura, Takashi, Kasai, Tatsuya
openaire   +3 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source