Results 31 to 40 of about 81,928 (262)

Detection and characterization of colorectal cancer by autofluorescence lifetime imaging on surgical specimens

open access: yesScientific Reports
Colorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report a characterization of the ex vivo autofluorescence lifetime fingerprint of colorectal tissues obtained ...
Alberto Ignacio Herrando   +11 more
doaj   +1 more source

Autofluorescence indexes as biomarkers for antiangiogenic loading dose outcome in diabetic macular edema

open access: yesTherapeutic Advances in Ophthalmology, 2020
Purpose: To evaluate the combination of fundus autofluorescence results with several clinical and structural variables into mathematical indexes to enhance their ability to predict visual and anatomical changes after the antivascular endothelial growth ...
Sergio E. Hernández Da Mota   +6 more
doaj   +1 more source

Near-infrared fluorescent imaging techniques for the detection and preservation of parathyroid glands during endocrine surgery

open access: yesInnovative Surgical Sciences, 2021
In over 30% of all thyroid surgeries, complications arise from transient and definitive hypoparathyroidism, underscoring the need for real-time identification and preservation of parathyroid glands (PGs).
Demarchi Marco Stefano   +3 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Microfluidic Production of Autofluorescent BSA Hydrogel Microspheres and Their Sequential Trapping for Fluorescence-Based On-Chip Permanganate Sensing

open access: yesSensors, 2020
Microfabrication technologies have extensively advanced over the past decades, realizing a variety of well-designed compact devices for material synthesis, separation, analysis, monitoring, sensing, and so on.
Linbo Liu   +4 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Autofluorescence of bone tissues [PDF]

open access: yesJournal of Clinical Pathology, 1967
The phenomenen of autofluorescence of bone is due primarily to the collagen itself rather than to incidental substances adsorbed on to it. Fluorescent microscopy is a convenient method of dating the different microanatomical components of bone, and in this respect the same conclusions can be reached from the study of microradiographs or autofluorescent
openaire   +2 more sources

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

open access: yesFEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

ATP‐Mediated Bidirectional Communication Between Natural and Artificial Cells for Controlled Bioreactions

open access: yesAdvanced Functional Materials, EarlyView.
Establishment of bidirectional communication behavior between natural and artificial cells. The first type of communication from natural dendritic cells to artificial polymeric cells was achieved via ATP release after cell lysis. The released ATP was taken up via gated UV‐controlled permeability increase of the polymeric membrane for performing ...
Ilona Kalte   +6 more
wiley   +1 more source

Prognostic significance of skin autofluorescence in patients undergoing maintenance hemodialysis

open access: yesКардиоСоматика
BACKGROUND: Maintenance hemodialysis remains the most common modality of renal replacement therapy, with a consistently high demand. Despite technological advances in recent decades, this high-cost treatment is still associated with a substantial ...
Darya Yu. Konovalova   +6 more
doaj   +1 more source

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