Results 51 to 60 of about 91,538 (308)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Parathyroid autofluorescence [PDF]

, 2019
Utilization of near infrared autofluorescence for intraoperative identification of parathyroid glands in surgery of thyroid and parathyroid ...
Serra, C (via Mendeley Data)
core   +1 more source

Detection and characterization of colorectal cancer by autofluorescence lifetime imaging on surgical specimens

Scientific Reports
Colorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report a characterization of the ex vivo autofluorescence lifetime fingerprint of colorectal tissues obtained ...
Alberto Ignacio Herrando, Laura M. Fernandez, José Azevedo, Pedro Vieira, Hugo Domingos, Antonio Galzerano, Vladislav Shcheslavskiy, Richard J. Heald, Amjad Parvaiz, Pedro Garcia da Silva, Mireia Castillo-Martin, João L. Lagarto   +11 more
doaj   +1 more source

Autofluorescence indexes as biomarkers for antiangiogenic loading dose outcome in diabetic macular edema

Therapeutic Advances in Ophthalmology, 2020
Purpose: To evaluate the combination of fundus autofluorescence results with several clinical and structural variables into mathematical indexes to enhance their ability to predict visual and anatomical changes after the antivascular endothelial growth ...
Sergio E. Hernández Da Mota, Francisco Béjar Cornejo, Marcela Esquivel Velázquez, Virgilio Lima Gómez, Gerardo González Saldívar, Ernesto Rodríguez Ayala, Raul Vélez-Montoya   +6 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Near-infrared fluorescent imaging techniques for the detection and preservation of parathyroid glands during endocrine surgery

Innovative Surgical Sciences, 2021
In over 30% of all thyroid surgeries, complications arise from transient and definitive hypoparathyroidism, underscoring the need for real-time identification and preservation of parathyroid glands (PGs).
Demarchi Marco Stefano, Karenovics Wolfram, Bédat Benoît, Triponez Frédéric   +3 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Spectral Characterization of Dictyostelium Autofluorescence [PDF]

, 2006
Dictyostelium discoideum is used extensively as a model organism for the study of chemotaxis. In recent years, an increasing number of studies of Dictyostelium chemotaxis have made use of fluorescence-based techniques.
Haastert, Peter J.M. van,, Engel, R., Visser, Antonie J.W.G., van Haastert, P.J.M., Visser, A.J.W.G., Engel, Ruchira,, van Haastert, P. J. M., Visser, Antonie J.W.G.,   +7 more
core   +2 more sources

Microfluidic Production of Autofluorescent BSA Hydrogel Microspheres and Their Sequential Trapping for Fluorescence-Based On-Chip Permanganate Sensing

Sensors, 2020
Microfabrication technologies have extensively advanced over the past decades, realizing a variety of well-designed compact devices for material synthesis, separation, analysis, monitoring, sensing, and so on.
Linbo Liu, Guangming Li, Nan Xiang, Xing Huang, Kota Shiba   +4 more
doaj   +1 more source

Autofluorescence of bone tissues [PDF]

Journal of Clinical Pathology, 1967
The phenomenen of autofluorescence of bone is due primarily to the collagen itself rather than to incidental substances adsorbed on to it. Fluorescent microscopy is a convenient method of dating the different microanatomical components of bone, and in this respect the same conclusions can be reached from the study of microradiographs or autofluorescent
openaire   +2 more sources