Results 71 to 80 of about 212,785 (269)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Automated Robotic Process Automation: A Self-Learning Approach

, 2019
Robotic Process Automation (RPA) recently gained a lot of attention, in both industry and academia. RPA embodies a collection of tools and techniques that allow business owners to automate repetitive manual tasks. The intrinsic value of RPA is beyond dispute, e.g., automation reduces errors and costs and thus allows us to increase the overall business ...
Junxiong Gao, Sebastiaan J. van Zelst, Xixi Lu 0001, Wil M. P. van der Aalst   +3 more
openaire   +2 more sources

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Tunable Automation in Automated Program Verification

CoRR
Automated verification tools based on SMT solvers have made significant progress in verifying complex software systems. However, these tools face a fundamental tension between automation and performance when dealing with quantifier instantiation -- the primary source of incompleteness and verification slowdown in SMT-based verifiers.
Alexander Y. Bai, Chris Hawblitzel, Andrea Lattuada 0001   +2 more
openaire   +2 more sources

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

FEA-Guided Toolpath Compensation for Robotic Machining: An Integrated CAD/CAM/CAE Framework for Enhanced Accuracy

Automation
Industrial robots offer flexibility and cost advantages in machining applications but suffer from limited structural stiffness and dynamic instability, leading to significant positional errors.
Vasileios D. Sagias, Michail Koutroumpousis, Constantinos Stergiou, Antonios Tsolakis, George Kioroglou, Paraskevi Zacharia   +5 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Design and Validation of a Low-Cost Automated Dip-Coater System for Laboratory Applications

Automation
Dip coating is a widely used laboratory method for depositing thin films and functional coatings. However, commercial dip-coaters remain costly and often exceed the needs of teaching labs and early-stage research.
Cesar H. Guzmán-Valdivia, Héctor R. Azcaray-Rivera, Arturo J. Martínez-Mata, Jorge A. Brizuela-Mendoza, Héctor M. Buenabad-Arias, Agustín Barrera-Sánchez, Andrés Blanco-Ortega   +6 more
doaj   +1 more source