Results 61 to 70 of about 547,940 (314)
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Validity of the Walked Distance Estimated by Wearable Devices in Stroke Individuals
Sensors, 2019 Background: Health professionals need valid devices to assess a stroke individual’s ability to walk. The aim was to evaluate the validity of the estimation of the walked distance by wearable devices and the impact of the sensor’s position in ...
Maxence Compagnat +6 more
doaj +1 more source
The American Journal of Bioethics, 2016 Taken as such, default rules do not intrude on autonomy, even if they influence people without persuading them. When default rules give people certain rights automatically (such as the right to vote), they promote autonomy for just that reason. And to the extent that default rules give people the freedom to focus on their most pressing concerns, they ...
openaire +2 more sources
The Concept of Precedent Autonomy
, 2002 Does respect for autonomy imply respect for precedent autonomy? The principle of respect for autonomy requires us to respect a competent patient's treatment preference, but not everyone agrees that it requires us to respect preferences formed earlier by ...
Davis, John K.
core +1 more source
Small RNA pathways in mammalian oocytes
FEBS Open Bio, EarlyView.Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
Intention, Autonomy, and Brain Events
, 2009 Informed consent is the practical expression of the doctrine of autonomy. But the very idea of autonomy and conscious free choice is undercut by the view that human beings react as their unconscious brain centres dictate, depending on factors that may or
Gillett, Grant
core +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Enhanced Solar Photovoltaic System Management and Integration: The Digital Twin Concept
SolarThe rapid acceptance of solar photovoltaic (PV) energy across various countries has created a pressing need for more coordinated approaches to the sustainable monitoring and maintenance of these widely distributed installations. To address this challenge,
Olufemi Olayiwola +3 more
doaj +1 more source
ON THE AUTONOMY OF THE HEART. [PDF]
JAMA: The Journal of the American Medical Association, 1894 In the last number of theAmerican Journal of Medical Sciences, Prof. I. M. Da Costa has published eight cases of weak heart, where the causation of neurasthenia and organic disorder, as valvular lesion or fatty degeneration had to be excluded. He is inclined to attribute this weakness to the influence of the heart ganglia.
openaire +1 more source