Results 241 to 250 of about 132,737 (315)

Cas9‐Rep fusion tethers donor DNA in vivo and boosts the efficiency of HDR‐mediated genome editing

Plant Biotechnology Journal, Volume 23, Issue 6, Page 2006-2017, June 2025.
Summary Genome editing based on the homology‐directed repair (HDR) pathway enables scar‐free and precise genetic manipulations. However, the low frequency of HDR hinders its application in plant genome editing. In this study, we engineered the fusion of Cas9 and a viral replication protein (Rep) as a molecular bridge to tether donor DNA in vivo, which ...
Zhentao Zhou, Jiahui Xiao, Shuai Yin, Yache Chen, Yang Yuan, Jianwei Zhang, Lizhong Xiong, Kabin Xie   +7 more
wiley   +1 more source

Biofunctionalized dissolvable hydrogel microbeads enable efficient characterization of native protein complexes. [PDF]

Nat Commun
Shao X, Tian M, Yin J, Duan H, Tian Y, Wang H, Xia C, Wang Z, Zhu Y, Wang Y, Chaihu L, Tan M, Wang H, Huang Y, Wang J, Wang G.   +15 more
europepmc   +1 more source

Recent Advances in Enzymes and Chemoenzymatic Synthesis of Tetrasaccharide Linkage Region of Proteoglycans

ChemBioChem, Volume 26, Issue 10, May 27, 2025.
The tetrasaccharide linkage region of proteoglycans is synthesized through a series of well‐controlled enzymatic reactions in nature. The expression, activity, and substrate profile of these biosynthetic enzymes and the latest development of chemoenzymatic synthesis of linkage region‐bearing glycopeptides are summarized in this review.
Po‐han Lin, Xuefei Huang
wiley   +1 more source

Adsorption Behavior of Avidin and Streptavidin on the Gold Electrodes Modified with Thiol Monolayers with Different Hydrophobicity and Polarity

, 1996
Qiang Chen, Jun‐ichi Anzai, Tetsuo Osa
openalex   +2 more sources

Polymerase delta-interacting protein 2 mediates brain vascular permeability by regulating ROS-mediated ZO-1 phosphorylation and localization at the interendothelial border. [PDF]

Cell Commun Signal
Wang K, Qu H, Hu R, Lassègue B, Eaton DC, Song C, Mu J, Griendling KK, Hernandes MS.   +8 more
europepmc   +1 more source

Low‐Intensity Pulsed Ultrasound Promotes Osteogenesis in Porous Titanium Alloys Through miR‐1187/BMP4 Pathway

The FASEB Journal, Volume 39, Issue 9, 15 May 2025.
The Schematic of LIPUS promotes osteogenesis of porous titanium alloys through inhibiting miR‐1187 and upregulating BMP4. ABSTRACT The repair of critical‐sized bone defects remains a significant clinical challenge. Low‐intensity pulsed ultrasound (LIPUS), in combination with porous titanium alloy (PTi) scaffolds, has emerged as a promising therapeutic ...
Limei Qin, Hongjuan Cao, Xiaohan Liu, Di Zhang, Lin Wu   +4 more
wiley   +1 more source

Feasibility of CD34-Positive Selection Using the Avidin-Biotin Immunoadsorption System.

, 1999
Shingo Yano, Osamu Asai, Akinori Kato, Hiroshi Watanabe, Mituji Katori, M Nagamine, Yuichi Yahagi, Nobuko Maki, Tadashi Kobayashi, Yasutaka Hoshi, Naoko Tajima, Yasunobu Kuraishi   +11 more
openalex   +2 more sources

Improvement of telomerase activity measurement by using a biotin addition primer and avidin magnetic beads.

, 1998
Marie Matsuda
openalex   +2 more sources

Microdetection of Nucleocapsid Proteins via Terahertz Chemical Microscope Using Aptamers. [PDF]

Sensors (Basel)
Ding X, Murakami M, Wang J, Inoue H, Kiwa T.   +4 more
europepmc   +1 more source

Deciphering neutrophil dynamics: Enhanced phagocytosis of elastic particles and impact on vascular-targeted carrier performance. [PDF]

Sci Adv
Lee JK, Guevara M, Akanbi OD, Hoff JD, Kupor D, Brannon ER, Eniola-Adefeso O.   +6 more
europepmc   +1 more source