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Towards a synthetic avidin mimic
Analytical and Bioanalytical Chemistry, 2011A series of streptavidin-mimicking molecularly imprinted polymers has been developed and evaluated for their biotin binding characteristics. A combination of molecular dynamics and NMR spectroscopy was used to examine potential polymer systems, in particular with the functional monomers methacrylic acid and 2-acrylamidopyridine.
Jesper, Wiklander +3 more
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Genetically engineered avidins and streptavidins
Cellular and Molecular Life Sciences, 2006Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to nanotechnology. (Strept)avidin-biotin technology relies on the extremely tight and specific affinity between (strept ...
O H, Laitinen +3 more
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Avidin Binds to Condensed Chromatin
Stain Technology, 1977The protein avidin has been conjugated with fluorescein isothiocyanate and then used to treat cells and chromosome preparations. Fluorescence microscopy reveals that this protein is bound by areas of the specimen containing condensed chromatin.
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(Strept)avidin Binds Glycoconjugates
Biochemistry (Moscow)Fluorescently labeled and conjugated (strept)avidins are widely used for imaging biotinylated molecules in immunological assays and histochemistry. We showed that besides biotin, these proteins bind glycans, including fragments of mammalian glycoproteins and glycolipids, in particular, ABO blood group antigens, oligolactosamines, and 6-O-sulfated ...
Nadezhda V, Shilova +6 more
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Avidin-Biotin Immobilisation Systems
1993Abstract Over the past two decades, the avid in–biotin system has evolved to be an extremely useful tool in the fields of biology, biochemistry, and biotechnology. The extraordinary affinity (Kd ∼ 10−15 M) of avid in (or its bacterial relative, streptavidin) for the vitamin biotin forms the basis for using this system.
Meir Wilchek, Edward A Bayer
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The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
2003Immunoenzyme methods can be enhanced by the use of the high affinity molecules, avidin and biotin. The binding of avidin to biotin is almost irreversible. By labeling a detection enzyme such as horseradish peroxidase with biotin, and a secondary antibody (reactive against the antigen detecting primary antibody) with biotin as well, these two compounds ...
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