Results 1 to 10 of about 236,402 (293)
Production of mutant streptavidin protein and investigation of its effect on the performance of streptavidin [PDF]
Iranian Journal of Basic Medical Sciences, 2023 Objective(s): Streptavidin is a versatile protein in cell science. The tetramer structure of streptavidin plays a key role in this binding, but this form interferes with some assays.
Elham Didevara +3 more
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Scalable production of monovalent streptavidin with high yield and purity [PDF]
Scientific ReportsThe tetrameric protein streptavidin has found ubiquitous use in a wide variety of applications due to its ability to bind biotin molecules with remarkable affinity (Kd of 10− 14 M).
Ian D. Wadsworth +5 more
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Streptavidins Coordinate Biotin Sequestration and Self‐Resistance Within a Biotin‐Pathway Antibiotic Network [PDF]
Advanced ScienceStreptavidin, the well‐known biotin‐binding protein extensively used in biotechnology, is naturally co‐produced in Streptomyces avidinii alongside stravidins—inhibitors of biotin biosynthesis.
Sumire Kurosawa +8 more
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Effective Tumor Targeting by EphA2-Agonist-Biotin-Streptavidin Conjugates
Molecules, 2021 We recently reported on a potent synthetic agent, 135H11, that selectively targets the receptor tyrosine kinase, EphA2. While 135H11 possesses a relatively high binding affinity for the ligand-binding domain of EphA2 (Kd~130 nM), receptor activation in ...
Parima Udompholkul +6 more
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Cancer Medicine, 2022 Background CARs are engineered receptors comprising an immunoglobulin single‐chain variable fragment (scFv) that identifies and binds to the target antigen, a transmembrane domain, and an intracellular T‐cell signaling domain.
Hui Lian +10 more
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Journal of Advanced Pharmaceutical Technology & Research, 2022 Nearly 95% of streptavidin which is expressed in Escherichia coli found as an inclusion body. Protein expressed in an inclusion body form requires further steps for the folding process related to its purification.
Toto Subroto +5 more
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Nanomaterials, 2021 The physiochemical properties of nanomaterials have a close relationship with their status in solution. As a result of its better simplicity than that of pre-assembled aggregates, the in situ assembly of nanomaterials has been integrated into the design ...
Yong Chang +4 more
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Biosensors, 2023 A scheme of modular competitive immunochromatography with an analyte-independent test strip and changeable specific immunoreactants has been proposed.
Lyubov V. Barshevskaya +3 more
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Current Issues in Molecular Biology, 2023 An immunoassay is an analytical test method in which analyte quantitation is based on signal responses generated as a consequence of an antibody–antigen interaction. They are the method of choice for the measurement of a large panel of diagnostic markers.
Amy H. A. Balzer +1 more
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Sensors, 2020 While label-free multiplex sensor technology enables “mixing and matching” of different capture molecules in principle, in practice this has been rarely (if ever) demonstrated. To fill this gap, we developed protocols for the preparation of mixed aptamer-
Alanna M. Klose, Benjamin L. Miller
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