Results 11 to 20 of about 318,069 (262)

Synthesis and Application of Albumin Nanoparticles Loaded with Prussian Blue Nanozymes

open access: yesColloids and Interfaces, 2022
Prussian blue nanozymes exhibit peroxidase-like catalytic activity and are therefore considered a stable and inexpensive alternative to natural peroxidases in the enzyme-linked immunosorbent assay (ELISA).
Pavel Khramtsov   +6 more
doaj   +1 more source

A Simple Biosensor Based on Streptavidin-HRP for the Detection of Bacteria Exploiting HRPs Molecular Surface Properties

open access: yesApplied Biosciences, 2023
Bacterial contamination of water and food sources is still a major source of diseases. Early detection of potential pathogens is key to prevent their spreading and severe health risks.
Filippo Savini   +3 more
doaj   +1 more source

Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines [PDF]

open access: yes, 1996
A powerful and potentially general approach to the targeting and crystallization of proteins on lipid interfaces through coordination of surface histidine residues to lipid-chelated divalent metal ions is presented.
Arnold, Frances H.   +7 more
core   +1 more source

Pre-hybridisation: an efficient way of suppressing endogenous biotin-binding activity inherent to biotin–streptavidin detection system [PDF]

open access: yes, 2014
Endogenous biotin or biotinylated protein binding activity is a major drawback to biotin-avidin/streptavidin detection system. The avidin/streptavidin conjugate used to detect the complex of the biotinylated secondary antibody and the primary antibody ...
Andrew Thompsett   +22 more
core   +2 more sources

SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY [PDF]

open access: yes, 2014
Indexación: Web of Science; Scielo.A hydrogen-terminated Si (111) surface was modified to form an aminoterminated monolayer for immobilization of streptavidin.
ARAYA, EYLEEN   +4 more
core   +2 more sources

Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry [PDF]

open access: yes, 2010
We developed a bioadhesive coating based on a synthetic peptide-conjugate (AK-cycloRGDfC]) which contains multiples of the arginyl-glycyl-aspartic acid (RGD) amino acid sequence.
Bert Nickel   +9 more
core   +3 more sources

A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles

open access: yesBiosensors, 2023
In this paper, we developed a sensitive, label-free and facile fluorescent strategy for detecting streptavidin (SA) based on double-hairpin DNA-templated copper nanoparticles (CuNPs) and terminal protection of small molecule-linked DNA. Herein, a special
Qiangsheng Xiao   +5 more
doaj   +1 more source

Hybridization Chain Reaction-Based Electrochemical Biosensors by Integrating the Advantages of Homogeneous Reaction and Heterogeneous Detection

open access: yesBiosensors, 2023
The conventional hybridization chain reaction (HCR)-based electrochemical biosensors usually require the immobilization of probes on the electrode surface.
Ning Xia   +5 more
doaj   +1 more source

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

open access: yesNanomaterials, 2019
The surface functionalization of magnetic nanoparticles improves their physicochemical properties and applicability in biomedicine. Natural polymers, including proteins, are prospective coatings capable of increasing the stability, biocompatibility, and ...
Pavel Khramtsov   +9 more
doaj   +1 more source

The Crystal Structure of Monovalent Streptavidin. [PDF]

open access: yes, 2016
The strong interaction between streptavidin (SA) and biotin is widely utilized in biotechnological applications. A SA variant, monovalent SA, was developed with a single and high affinity biotin-binding site within the intact tetramer.
Biswas, Sangita   +3 more
core   +1 more source

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