Results 21 to 30 of about 236,402 (293)

A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles

open access: yesBiosensors, 2023
In this paper, we developed a sensitive, label-free and facile fluorescent strategy for detecting streptavidin (SA) based on double-hairpin DNA-templated copper nanoparticles (CuNPs) and terminal protection of small molecule-linked DNA. Herein, a special
Qiangsheng Xiao   +5 more
doaj   +1 more source

Streptavidin Coverage on Biotinylated Surfaces [PDF]

open access: yesACS Applied Materials & Interfaces, 2021
Biosensors and other biological platform technologies require the functionalization of their surface with receptors to enhance affinity and selectivity. Control over the functionalization density is required to tune the platform's properties. Streptavidin (SAv) monolayers are widely used to immobilize biotinylated proteins, receptors, and DNA.
P. H. Erik Hamming, Jurriaan Huskens
openaire   +3 more sources

Immobilization of biotinylated antibodies through streptavidin binding aptamer [PDF]

open access: yes, 2023
Liquid biopsy approaches are powerful strategies that potentially allow the diagnosis and prognosis of a number of diseases. The field is continuously and rapidly growing, encouraging the discovery of novel predictory biomarkers.
Laura , Sola   +4 more
core   +2 more sources

Hybridization Chain Reaction-Based Electrochemical Biosensors by Integrating the Advantages of Homogeneous Reaction and Heterogeneous Detection

open access: yesBiosensors, 2023
The conventional hybridization chain reaction (HCR)-based electrochemical biosensors usually require the immobilization of probes on the electrode surface.
Ning Xia   +5 more
doaj   +1 more source

On the Dye-Protein Interaction of TMR-Biocytin – Streptavidin Complex: Molecular Simulations and Time-Resolved Fluorescence Quenching [PDF]

open access: yes, 2023
The interaction of a fluorophore and a protein, as a type of chemical-protein interactions (CPIs), is important toward studying physical properties of a protein through spectroscopy.
Jang Mok, Yoo   +3 more
core   +2 more sources

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

open access: yesNanomaterials, 2019
The surface functionalization of magnetic nanoparticles improves their physicochemical properties and applicability in biomedicine. Natural polymers, including proteins, are prospective coatings capable of increasing the stability, biocompatibility, and ...
Pavel Khramtsov   +9 more
doaj   +1 more source

Fiber Optic Particle Plasmon Resonance-Based Immunoassay Using a Novel Multi-Microchannel Biochip

open access: yesSensors, 2020
A novel multi-microchannel biochip fiber-optic particle plasmon resonance (FOPPR) sensor system for the simultaneous detection of multiple samples.
Chang-Yue Chiang   +2 more
doaj   +1 more source

Nanomechanics of Streptavidin Hubs for Molecular Materials [PDF]

open access: yes, 2014
A new strategy is reported for creating protein-based nanomaterials by genetically fusing large polypeptides to monomeric streptavidin and exploiting the propensity of streptavidin monomers(SM) to self-assemble into stable tetramers.
Benedetti, Fabrizio   +5 more
core   +2 more sources

Novel approaches for the creation of artificial metalloenzymes [PDF]

open access: yes, 2013
Artificial metalloenzymes can be defined as the fusion of chemo- and enzymatic catalysts by insertion of non-specific achiral catalytic moieties into the chiral environment of a protein cavity, thereby broadening the scope of both fields.
Pereira Nogueira, Elisa Sofia
core   +1 more source

Spontaneous Dissociation of Streptavidin from Biotinylated Double Stranded DNA [PDF]

open access: yes, 2017
The binding of streptavidin to biotin is one of the strongest non-covalent interactions observed in nature. However, we have observed that streptavidin rapidly dissociates from biotinylated double stranded DNA in the presence of free biotin. In contrast,
Kennedy, Michelle
core   +6 more sources

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