Results 41 to 50 of about 318,069 (262)

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

BioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson, Jason S. King, Robert H. Insall   +2 more
doaj   +1 more source

Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation

BioTechniques, 2010
Detection of protein-protein interactions in cells is crucial for understanding the biological functions of proteins, including their roles in signal transduction.
Cai Huang, Ken Jacobson
doaj   +1 more source

Microspinning: Local Surface Mixing via Rotation of Magnetic Microparticles for Efficient Small-Volume Bioassays [PDF]

, 2020
The need for high-throughput screening has led to the miniaturization of the reaction volume of the chamber in bioassays. As the reactor gets smaller, surface tension dominates the gravitational or inertial force, and mixing efficiency decreases in small-
Cho, Seongkyu, Jeong, Hyun Yong, Jeong, Yunjin, Kang, Taehun, Kim, Jiyun, Kim, Min Chang, Kim, Su Deok, Koo, Jeong Woo, Kwon, Sunghoon, Lee, Amos Chungwon, Lee, Changhee, Lee, Daewon, Oh, Dong Yoon, Song, Seo Woo   +13 more
core   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Synthesis of Biotin Linkers with the Activated Triple Bond Donor [p-(N-propynoylamino)toluic Acid] (PATA) for Efficient Biotinylation of Peptides and Oligonucleotides

Molecules, 2012
Biotin is an important molecule for modern biological studies including, e.g., cellular transport. Its exclusive affinity to fluorescent streptavidin/avidin proteins allows ready and specific detection.
Martina Jezowska, Joanna Romanowska, Burcu Bestas, Ulf Tedebark, Malgorzata Honcharenko   +4 more
doaj   +1 more source

Layer guided-acoustic plate mode biosensors for monitoring MHC-peptide interactions [PDF]

, 2006
The transduction signals from the immobilisation of a class I heavy chain, HLA-A2, on a layer guided acoustic plate mode device, followed by binding of beta(2)-microglobulin and subsequent selective binding of a target peptide are ...
Dodi, I. A., Evans, Carl, McHale, Glen, Newton, Michael, Paston, S. J., Percival, Carl, Rees, Robert, Stanley, Simon   +7 more
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

The case for trypsin release of affinity-selected phages

BioTechniques, 2010
Libraries of phages displaying diverse peptides are typically surveyed by affinity selection, using immobilized biomolecules as selectors. After exposing the library to the selector and washing away unbound phages, the bound phages are enriched for ...
William D. Thomas, George P. Smith
doaj   +1 more source

Development of the Method to Produce Functionally Active Recombinant Streptavidin in Escherichia coli Cells [PDF]

Журнал Сибирского федерального университета: Серия Биология, 2020
Streptavidin is a homotetrameric protein produced by Streptomyces avidinii, each subunit of which binds biotin (vitamin H), forming a stable complex (Kd = 10-15 М).
Eugenia E. Bashmakova, Alexander N. Kudryavtsev, Ludmila A. Frank   +2 more
doaj   +1 more source

Reversible positioning of single molecules inside zero-mode waveguides [PDF]

, 2014
We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to
Foquet, Mathieu, Korlach, Jonas, Larkin, Joseph, Turner, Stephen W., Wanunu, Meni   +4 more
core   +2 more sources