Results 41 to 50 of about 236,402 (293)

An Artificial Peroxidase based on the Biotin-Streptavidin Technology that Rivals the Efficiency of Natural Peroxidases [PDF]

, 2023
Horseradish peroxidase (HRP) is an archetypal heme-containing metalloenzyme that uses peroxide to oxidize various substrates. Capitalizing on a well-established catalytic mechanism, diverse peroxidase mimics have been widely investigated and optimized ...
Nico V., Igareta, Manjistha, Mukherjee, Dilbirin, Üzümcü, Thomas R., Ward, Alec H., Follmer, Roman P., jakob, Timm, Maier, Valerie, Waser   +7 more
core   +2 more sources

Insights into the structure of mature streptavidin C1 from Streptomyces cinnamonensis reveal the self-binding of the extension C-terminal peptide to biotin-binding sites

IUCrJ, 2021
The members of the avidin protein family are well known for their high affinity towards d-biotin and their structural stability. These properties make avidins a valuable tool for various biotechnological applications.
Byeong Jun Jeon, Sulhee Kim, Min-Seok Kim, Ji-Ho Lee, Beom Seok Kim, Kwang Yeon Hwang   +5 more
doaj   +1 more source

Sensing with silicon nanowire field-effect transistors [PDF]

, 2011
All experiments presented in this thesis are focusing on the development of a reliable and stable sensing platform, which allows converting a (bio-) chemical signal into an electrical, processable one.
Knopfmacher, Oren
core   +1 more source

An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles

Frontiers in Bioengineering and Biotechnology
Helicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of H. pylori is of great significance for the prevention of gastric cancer.
Xiaojuan You, Xiaojuan You, Xiaojuan You, Xiaojuan You, Mingyi Shao, Mingyi Shao, Huadong Wang, Rui Zhu, Xinwei Liu, Lei Dong, Yuesheng Gong, Yongwei Li, Yongwei Li   +12 more
doaj   +1 more source

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

BioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson, Jason S. King, Robert H. Insall   +2 more
doaj   +1 more source

Streptavidin-Conjugated DNA for the Boronate Affinity-Based Detection of Poly(ADP-Ribose) Polymerase-1 with Improved Sensitivity

Biosensors, 2023
This work reports the development of a fluorescence method for the detection of poly(ADP-ribose) polymerase-1 (PARP1), in which a phenylboronic acid-modified fluorescein isothiocyanate dye (FITC-PBA) was used to recognize the formed poly(ADP-ribose) (PAR)
Fengli Gao, Gang Liu, Yishu Qiao, Xiuwen Dong, Lin Liu   +4 more
doaj   +1 more source

Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation

BioTechniques, 2010
Detection of protein-protein interactions in cells is crucial for understanding the biological functions of proteins, including their roles in signal transduction.
Cai Huang, Ken Jacobson
doaj   +1 more source

Monovalent Streptavidin that Senses Oligonucleotides [PDF]

Angewandte Chemie, 2013
We report a straightforward chemical route to monovalent streptavidin, a valuable reagent for imaging. The one-step process is based on a (tris)biotinylated-oligonucleotide blocking three of streptavidin’s four biotin binding sites. Further, the complex is highly sensitive to single-base differences - whereby perfectly matched oligonucleotides trigger ...
Steven K, Taylor, Jingxian, Wang, Natasa, Kostic, Milan N, Stojanovic   +3 more
openaire   +2 more sources

Engineering artificial metalloenzymes based on biotin-streptavidin technology for DNA recognition and asymmetric transfer hydrogenation catalysis [PDF]

, 2012
: Metalloproteins occupy a central place among all proteins since they represent nearly half of all proteins in nature. Metalloenzymes catalyze important biological processes (photosynthesis, respiration, water oxidation) and other chemical reactions ...
Zimbron, Malcolm Jeremy
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source