Results 211 to 220 of about 90,586 (267)
Poly(ethylene glycol)–avidin bioconjugates: suitable candidates for tumor pretargeting
Journal of Controlled Release, 2002 Avidin-poly(ethylene glycol) (PEG) conjugates were obtained by derivatization of about 10% of the protein amino groups (four amino groups per protein molecule) with linear 5 kDa PEG or branched 10 or 20 kDa PEGs.
Paolo Caliceti +2 more
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Nature, 1958
AVIDIN has been shown to bind the vitamin biotin, but the mechanism of binding is still unknown. Modification of the avidin molecule by acetylation, esterifieation or by blocking the amide, guanidyl, imidazole and phenolic groups did not result in a loss of its ability to bind biotin1.
D V, SIVA SANKAR +3 more
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AVIDIN has been shown to bind the vitamin biotin, but the mechanism of binding is still unknown. Modification of the avidin molecule by acetylation, esterifieation or by blocking the amide, guanidyl, imidazole and phenolic groups did not result in a loss of its ability to bind biotin1.
D V, SIVA SANKAR +3 more
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Avidin. II. Composition and mode of action of avidin A
Archives of Biochemistry and Biophysics, 1952Abstract Avidin A is a glycoprotein containing mannose and hexosamine. Data concerning its amino acid composition and its amino, acidic, and basic groups are given. Avidin XA, while similar in most analytical respects, contains galactose also. A variety of chemical modification reactions did not appreciably affect the biotin-binding activity of ...
H, FRAENKEL-CONRAT +2 more
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DNA condensation by high-affinity interaction with avidin
Journal of Molecular Recognition, 2004 Avidin, the basic biotin-binding glycoprotein from chicken egg white, is known to interact with DNA, whereas streptavidin, its neutral non-glycosylated bacterial analog, does not. In the present study we investigated the DNA-binding properties of avidin.
Margherita Morpurgo
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Recombinant NeutraLite Avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties [PDF]
FEBS Letters, 2000 A recombinant non-glycosylated and acidic form of avidin was designed and expressed in soluble form in baculovirus-infected insect cells. The mutations were based on the same principles that guided the design of the chemically and enzymatically modified ...
Edward Bayer +2 more
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Avidin-HRP conjugates in biotin-avidin immunoenzyme cytochemistry
Histochemistry, 1986Avidin-HRP conjugates were prepared, analysed and tested for avidin-biotin immunocytochemistry. Suitable biotinylation of enzymes, antigens and antibody was obtained by reacting biotin at equimolar ratio to epsilon aminogroups in proteins. The avidin-biotin interaction was used for immunocytochemical detection of phenomena in the field of immunology, i.
D M, Boorsma +2 more
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Hormonal Properties of Avidin-Biotinylinsulin and Avidin-Biotinylcorticotropin Complexes
Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1981In connection with the development of affinity columns (based on the avidin-biotin interaction) for retrieval of peptide and protein hormone receptors, the hormonal properties of a number of avidin-biotinylinsulin and avidin-bioinylcorticotropin complexes were examined.
F M, Finn +3 more
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Avidin–Biotin Technology: Preparation of Avidin Conjugates
2003The second step required in the application of avidin-biotin technology is to prepare an appropriate avidin-associated probe or probes for the desired application (for general reviews, see refs. 1 and 2). For example, a fluorescent form of avidin can be used for fluorescence microscopy, fluorescence-activated cell sorting, and in some cases, for ...
E A, Bayer, M, Wilchek
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Preparation of Avidin Conjugates
1996The high-affinity avidin-biotin system has found applications in different fields of biotechnology, including immunoassays, histochemistry, affinity chromatography, and drug delivery, to name a few. A brief description of avidin and avidin-like molecules, streptavidin, deglycosylated avidin, and NeutraLite avidin is presented in the Chapter 2.
R P, Haugland, M K, Bhalgat
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The avidin-biotin complex in immunology
Immunology Today, 1984The tenacious interaction between the vitamin biotin and theglycoprotein avidin has been applied in many immunological systems for the isolation, localization and visualization of various antigens as well as drug delivery, lymphocyte stimulation and immunoassays.
M, Wilchek, E A, Bayer
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