Results 51 to 60 of about 27,854 (263)

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

DuctilityPerformanceandUltimateAxialCompressionRatioofSteelReinforced ConcreteColumnswithT-shapedSteelCross-section

工程科学与技术, 2014
:Axial compression ratio is a critical factor which affects the structure’s ductility and resistance to progressive collapse.Through 12 specimens of T-shaped steel reinforced concrete columns under cyclic load loading test, the failure mode,hysteretic ...
曾磊, 吴园园, 石峰, 胡志文
doaj  

Experimental Study on and Finite Element Analysis of the Axial Compression Bearing Capacity of a UHPC Transfer Device for Pre-Stressed Anti-Floating Anchor Rods

Buildings
A new UHPC pre-stress transfer device is proposed for pre-stressed anti-floating anchor rods. To investigate the axial compression performance of the device during pre-stressing, physical experiments and a finite element verification were conducted on ...
Yi Zhu, Yi Li, Zhen-Xing Tang, Pei-Lin Liu, Zhuo Yang   +4 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Research on Axial Compression Performance of Waste Fiber Recycled Concrete Column

IOP Conference Series: Earth and Environmental Science, 2020
Abstract In order to investigate the compressive performance of waste fiber recycled concrete(WFRC) columns under axial compression, this paper used ABAQUS finite element software(FEM) to establish 8 models of WFRC columns, taking the length of waste fibers, volume of waste fibers and replacement rate of recycled aggregate as variables ...
Jinghai Zhou, Li Zhao, Yichen Wang, Qi Zhao   +3 more
openaire   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Study on the Axial Compression Performance of UHPC-SRC Short Columns

Buildings
This study proposes a novel UHPC-SRC short column structure that adopts ultra-high performance concrete (UHPC) as a permanent formwork, combined with longitudinal reinforcement, stirrups, and H-shaped steel. The design aims to enhance construction efficiency and sustainability while maintaining excellent mechanical performance.
Ruochen Wang, Kun Wang, Xiaobin Wei
openaire   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source