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Neutrophil granulation stained with May Gruenwald Giemsa or pure Azure B-eosin Y quantified by image analysis

Blut, 1989
Neutrophil granulation was quantified after staining with May Grunwald Giemsa or with the pure dyes Azure B and eosin Y. Spinner slides of the buffy coat of 3 normal subjects and 14 persons with different grades of toxic granulation were studied. Morphometric parameters were measured using an image analysis computer (Texture Analysis System, Leitz ...
W. Huiges   +4 more
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A general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver

Analytical Biochemistry, 1990
A sensitive method has been developed for the visualization of nonradiolabeled glycosaminoglycan oligosaccharides resolved by polyacrylamide gel electrophoresis using fixation with azure A followed by staining with ammoniacal silver. This method, which can detect as little as 1-2 ng of a single oligosaccharide species, can be used to stain a few ...
Malcolm Lyon, John T. Gallagher
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The Use of a Basic Dye (Azure a or Toluidme Blue) Plus a Cationic Surfactant for Selective Staining of RNA: a Technical and Mechanistic Study

Stain Technology, 1975
Selective purple staining of RNA-rich structures such as basophilic cytoplasms of exocrine pancreas and plasma cells, Nissl substance, and nucleoli was achieved by treating tissue sections as follows. Stain dewaxed sections for 1/2 hour in a dyebath containing 0.1% w/v axure A or toluidine blue and 1% cationic surfactant (Hyamine 2389, a 50% w/v ...
Richard W. Horobin   +2 more
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Azure B-eosin staining of blood cells: the effects of variation in stain formulation and staining technique on stain performance.

Analytical and quantitative cytology, 1980
A quantitative study of azure B-eosin-stained blood cells is reported. The effects of variation in stain formulation and staining technique on the binding of azure B and eosin by acidophilic, basophilic and neutrophilic substrates were measured by scanning microdensitometry.
S A, Bentley   +3 more
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Azure a-Schiff, Alcian Blue, HIO4-Schiff, Naphthol Yellow S—A Sequential Staining Method for Paraffin Sections

Stain Technology, 1966
Paraffin sections from tissue fixed 4-12 hr in 10% formalin containing 0.5% cetyl pyridinium chloride, and washed 2 hr, were stained as follows: (1) Hydrolyze in 5 N HCl at room temperature for 8.5-9 min, or use standard Feulgen hydrolysis at 60 C. (2) Stain in azure A-Schiff, 0.5% in bisulfite bleach (1 N HCl, 5; 10% Na2S2O5, 5; and distilled water 90—
openaire   +3 more sources

Endoscopic observation of the gastric mucus in vivo stained with azure A.

The American journal of gastroenterology, 1976
Gastric mucus was stained with Azure A, a cationic dye, which had the highest affinity with macromolecular constituents of the mucus, under such conditions as 0.2% Azure A-0.5% NaHCO3 solution (pH 8.1) in dye concentration, staining for ten minutes, 37 degrees C in reaction temperature and the salt concentration and ionic strength below 6.0 x 10(-2 ...
T, Kamada   +4 more
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Mallory's Phloxine B-Methylene Blue-Azure II Stain Emphasizes Elastin and Collagen Bundles in Epoxy Embedded Lung

Stain Technology, 1988
A version of Mallory's phloxine-methylene blue-azure II technique suitable for large epoxy sections is described. Phloxine B (C.I. 45410) and a yellow-green interference filter (546-548 nm transmission) combine to give high contrast monochrome images. By comparing light micrographs of lung parenchyma entirely unstained or stained only with phloxine B ...
openaire   +3 more sources

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