Results 81 to 90 of about 2,475 (111)

Quantification and Characterization of Glycosaminoglycans at the Nanogram Level by a Combined Azure A-Silver Staining in Agarose Gels

Analytical Biochemistry, 1994
A rapid, sensitive, and nonradioactive method has been developed for the quantification and characterization of glycosaminoglycans. The method is based on the separation of different types of glycosaminoglycans in agarose gel and subsequent fixation and staining with the cationic dye azure A, followed by silver enhancement.
C.H.A. van de Lest, Elly M. M. Versteeg, T. H. van Kuppevelt, J.H. Veerkamp   +3 more
openaire   +3 more sources

Lower Azure B Methylene Blue Ratios in Giemsa Type Blood and Malaria Stains

Stain Technology, 1978
Starting from ancient reports that rare samples of methylene blue were apparently sufficiently contaminated with azures to give red plasmodial and red purple nuclear chromatin in Chenzinsky type methylene blue eosin stains, it was decided to determine how little azure B would suffice for such staining in methylene blue eosin stains.
Angelo Russo, R. D. Lillie, P. T. Donaldson   +2 more
openaire   +2 more sources

Staining of Small Lymphoid Nucleoli in Paraffin Sections by Aniline-Azure B

Stain Technology, 1966
To see small lymphoid nucleoli clearly in 1-2 μ paraffin sections, the staining of contiguous chromatin masses in the nucleus was suppressed by a hydrolysis-aniline blocking sequence, which produces aldehyde from DNA, and attaches aniline to that aldehyde to make a diphenamine base, thus reducing the acidity of the chromatin and its affinity for basic ...
openaire   +3 more sources

Plant Cuticle Staining with Bismarck Brown Y and Azure B or Toluidine Blue O before Paraffin Extraction [PDF]

Biotechnic & Histochemistry, 1996
Transverse paraffin sections of mature greenwood stems of rose (Rosa x hybrida) and flowering dogwood (Cornus florida L.) were stained with Bismarck brown followed by azure B or toluidine blue 0. The Bismarck brown was replaced by thiazin dye metachromasia in all structures except the cuticle which remained brown or yellow.
Graham, E. T., Joshi, P. A.
openaire   +2 more sources

Microspectrophotometric Studies of Romanowsky Stained Blood Cells. III. The Action of Methylene Blue and Azure B

Stain Technology, 1984
The performances of two standardized Romanowsky stains (azure B/eosin and azure B/methylene blue/eosin) have been compared with each other and with a methylene blue/eosin stain. Visible-light absorbance spectra of various hematological substrates have been measured.
William Galbraith, Paul N. Marshall
openaire   +3 more sources

A Simple Methylene Blue-Azure Ii-Basic Fuchsin Stain for Epoxy-Embedded Tissue Sections

Stain Technology, 1974
One micron-thick sections of tissues fixed in glutaraldehyde, or in glutaraldehyde followed by osmium tetroxide, and embedded in a variety of plastic resins were stained in a methylene blue-azure II solution at 65 C, then counterstained in 0.05% basic fuchsin in 2.5% ethanol at room temperature (24 C). Considerable variation was found in methylene blue-
Fred E. Pittman, Charles D. Humphrey
openaire   +3 more sources

Which granules can be stained with azure B and eosin?

Blood cells, 1990
The standardized stain composed of pure azure B and eosin, as published by Wittekind and colleagues in 1986, demonstrated granules in neutrophilic leucocytes that were much coarser than those seen after staining with conventional Romanowsky-Giemsa methods. These granules belong to at least two classes.
H, Kurz, G, Leder, O, Leder
openaire   +2 more sources

Sulfation-Hematoxylin-Azure B as an Alternative to Periodic Acid-Schiff Staining

Laboratory Medicine, 1989
Gentle sulfation of formalin-fixed tissue sections followed by staining in a hematoxylin-azure B sequence results in bright blue staining of tissue elements that stain with the periodic acid-Schiff reaction—especially basement membranes. The stain is relatively rapid, simple, and permanent, is performed with inexpensive, readily available laboratory ...
openaire   +2 more sources

Kann Azur B-osin die May-Gr�nwald-Giemsa-F�rbung ersetzen?

Blut, 1976
A new method is described for staining blood and bone marrow smears. It is characterized by the presence of only two dyes, purified azure B and eosin in methanol, as stock solutions. Staining results are equivalent to those obtained by using the traditional dye mixtures according to May and Grunwald, Giemsa, Leishman or Wright.
Wittekind D, Vera Kretschmer, Löhr W
openaire   +3 more sources

Heparins, Low-Molecular-Weight Heparins, and Other Glycosaminoglycans Analyzed by Agarose Gel Electrophoresis and Azure A-Silver Staining

Seminars in Thrombosis and Hemostasis, 1997
A sensitive, nonradioactive azure A-silver staining method combining agarose gel electrophoresis was established and evaluated. Unfractionated heparins (UFHs), low-molecular-weight heparins (LMWHs), heparan sulfate (HS), chondroitin sulfate A (CSA), dermatan sulfate (DS), keratan sulfate (KS), and hyaluronic acid (HA) were analyzed. The detection limit
Wang Lianchun, Reinhard Malsch, Job Harenberg   +2 more
openaire   +3 more sources