Results 31 to 40 of about 603 (111)

Evaluation of the ELITe InGenius and Bordetella ELITe MGB Kit for the detection and identification of B. pertussis, B. parapertussis and B. holmesii

Diagnostic Microbiology and Infectious Disease
Bordetella pertussis is the causative pathogen of whooping cough or pertussis, a contagious respiratory disease. Aside from serodiagnosis, laboratory confirmation of pertussis is done through PCR, as B. pertussis is difficult to culture. The ELITe InGenius instrument (ELITechGroup, France) with accompanying Bordetella ELITe MGB Kit was evaluated ...
Helena Martini, Oriane Soetens, Thomas Demuyser, Eveline Van Honacker, Denis Piérard, Ingrid Wybo   +5 more
openaire   +4 more sources

Conserved Patterns of Symmetric Inversion in the Genome Evolution of Bordetella Respiratory Pathogens

mSystems, 2019
Whooping cough (pertussis), primarily caused by Bordetella pertussis, has resurged in the United States, and circulating strains exhibit considerable chromosome structural fluidity in the form of rearrangement and deletion.
Michael R. Weigand, Yanhui Peng, Dhwani Batra, Mark Burroughs, Jamie K. Davis, Kristen Knipe, Vladimir N. Loparev, Taccara Johnson, Phalasy Juieng, Lori A. Rowe, Mili Sheth, Kevin Tang, Yvette Unoarumhi, Margaret M. Williams, M. Lucia Tondella   +14 more
doaj   +3 more sources

Laboratory-based surveillance of pertussis using multitarget real-time PCR in Japan: evidence for Bordetella pertussis infection in preteens and teens

New Microbes and New Infections, 2015
Between January 2013 and December 2014, we conducted laboratory-based surveillance of pertussis using multitarget real-time PCR, which discriminates among Bordetella pertussis, Bordetella parapertussis, Bordetella holmesii and Mycoplasma pneumoniae.
K. Kamachi, S. Yoshino, C. Katsukawa, N. Otsuka, Y. Hiramatsu, K. Shibayama   +5 more
doaj   +1 more source

A real-time PCR assay with improved specificity for detection and discrimination of all clinically relevant Bordetella species by the presence and distribution of three Insertion Sequence elements

BMC Research Notes, 2011
Background In Dutch laboratories molecular detection of B. pertussis and B. parapertussis is commonly based on insertion sequences IS481 and IS1001, respectively. Both IS elements are more widely spread among Bordetella species. Both Bordetella holmesii,
Ossewaarde Jacobus M, Buitenwerf Johannes, Roorda Lieuwe, van der Zee Anneke   +3 more
doaj   +1 more source

Culture‐negative group B streptococcal pericarditis: A case report and literature review of the diagnostic use of polymerase chain reaction

Clinical Case Reports, Volume 7, Issue 3, Page 509-514, March 2019., 2019
Although conventional microbiology cultures may be negative, polymerase chain reaction (PCR) can effectively identify both typical and atypical microorganisms. With careful interpretation, PCR could become the gold‐standard diagnostic test for culture‐negative bacterial pericarditis.
Takahiro Tsushima, Natsuko Ishii, Suguru Matsuzaka, Keith Armitage, Kiyofumi Ohkusu, Ivor Cammack, Akira Yamada, Yuichiro Mori, Shunsuke Sasaki, Kentaro Hayashi, Yoshimoto Serizawa   +10 more
wiley   +1 more source

Initial modelling and updates on cost effectiveness from the first 10 years of a spleen registry

Australian and New Zealand Journal of Public Health, Volume 42, Issue 5, Page 463-466, October 2018., 2018
Abstract Objective: To validate our estimates from our original model and re‐evaluate the cost‐effectiveness of Spleen Australia, the Australian post‐splenectomy registry, using our original model with updated model parameters based on advances in the literature and experience of the registry over the past decade. Methods: We revisited a decision model
Sarah Luu, Penelope Jones, Ian Woolley, Denis Spelman, Lisa Gold   +4 more
wiley   +1 more source

Bordetella petrii Infection with Long-lasting Persistence in Human

Emerging Infectious Diseases, 2011
We report the repeated isolation of Bordetella petrii in the sputum of a 79-year-old female patient with diffuse bronchiectasis and persistence of the bacterium for >1 year.
Alain Le Coustumier, Elisabeth Njamkepo, Vincent Cattoir, Sophie Guillot, Nicole Guiso   +4 more
doaj   +1 more source

Detection and discrimination of B. pertussis and B. holmesii by real-time PCR targeting IS481 using a beacon probe and probe-target melting analysis

Molecular and Cellular Probes, 2003
A beacon probe was designed to detect one of the two documented single nucleotide changes in IS481 target allele of Bordetella holmesii genome as compared to Bordetella pertussis. PCR amplified product targeting a region of IS481 in presence of the probe was subjected to a post-PCR hybridization and melting cycle. Hybrid of the probe with B.
openaire   +2 more sources

Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real‐Time Polymerase Chain Reaction

Canadian Journal of Infectious Diseases and Medical Microbiology, Volume 25, Issue 4, Page 217-221, 2014., 2014
BACKGROUND: Bordetella pertussis infections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detect B pertussis are typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificity. METHODS: A novel B pertussis real‐time PCR assay based on the porin
Mohammad Rubayet Hasan, Rusung Tan, Ghada N Al-Rawahi, Eva Thomas, Peter Tilley   +4 more
wiley   +1 more source

Emerging occurrence of Gambierdiscus carolinianus in the Azores: The northernmost Atlantic record

Limnology and Oceanography Letters, Volume 11, Issue 4, July 2026.
Abstract The dinoflagellate genus Gambierdiscus was detected for the first time in the Azores Archipelago, representing its northernmost record to date (> 39°N). Two strains were isolated from macroalgal substrates on Corvo and Faial Islands during the summer of 2024, coinciding with a marine heatwave in which sea‐surface temperature anomalies exceeded
Miguel Barbosa, Catarina Churro, Inês Gomes, Sergei Danchenko, Maria Filomena Caeiro, Pedro Afonso, Ana Amorim, Pedro Reis Costa   +7 more
wiley   +1 more source