Results 71 to 80 of about 664 (136)

Microbial Engineering for Production of N‐Functionalized Amino Acids and Amines

open access: yesBiotechnology Journal, Volume 15, Issue 7, July 2020., 2020
Microbial production of N‐functionalized amines and amino acids is reviewed in terms of recent advances and applications and the prospects of developing new fermentation processes. N‐functionalizing enzymes and strain engineering are focused here. Abstract N‐functionalized amines play important roles in nature and occur, for example, in the antibiotic ...
Melanie Mindt   +3 more
wiley   +1 more source

Impact of CRISPR interference on strain development in biotechnology

open access: yesBiotechnology and Applied Biochemistry, Volume 67, Issue 1, Page 7-21, January/February 2020., 2020
CRISPR interference drives metabolic engineering in biotechnology. Abstract Genetic perturbation systems are of great interest to redirect metabolic fluxes for value‐added production, as well as genetic screening for the development of new drugs, or to identify new targets for biotechnological applications. Here, we review CRISPR interference (CRISPRi),
Kerstin Schultenkämper   +2 more
wiley   +1 more source

Cloning, expression, and sequence analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene [PDF]

open access: yesJournal of Bacteriology, 1992
The gene (mdh) coding for methanol dehydrogenase (MDH) of thermotolerant, methylotroph Bacillus methanolicus C1 has been cloned and sequenced. The deduced amino acid sequence of the mdh gene exhibited similarity to those of five other alcohol dehydrogenase (type III) enzymes, which are distinct from the long-chain zinc-containing (type I) or short ...
de Vries, Gerhard   +3 more
openaire   +3 more sources

Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria

open access: yesMicroorganisms, 2015
Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy
Tami L. McTaggart   +7 more
doaj   +1 more source

Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae

open access: yesMicroorganisms, 2018
Although Escherichia coli and Bacillus subtilis are the most prominent bacterial hosts for recombinant protein production by far, additional species are being explored as alternatives for production of difficult-to-express proteins.
Eivind B. Drejer   +3 more
doaj   +1 more source

Engineering Methylotrophic Yeasts as Cell Factories for Chemical Production Using Methanol as a Feedstock

open access: yesGCB Bioenergy, Volume 17, Issue 8, August 2025.
Methanol, a sustainable and abundant one‐carbon feedstock, can be utilized by natural methylotrophic yeasts to synthesize valuable chemicals. However, methanol‐based manufacturing with these yeasts faces several challenges, including low assimilation efficiency, carbon loss, and methanol toxicity. This review summarizes and discusses recent engineering
Shuxian Wang   +4 more
wiley   +1 more source

High‐quality genome of a novel Thermosynechococcaceae species from Namibia and characterization of its protein expression patterns at elevated temperatures

open access: yesMicrobiologyOpen, Volume 13, Issue 5, October 2024.
In this study, the genome of a new thermophilic cyanobacterium, Thermosynechococcaceae cyanobacterium sp. Okahandja, isolated from a hot spring near Okahandja in Namibia, was sequenced. Additionally, cultivations were conducted at elevated temperatures of 40, 50, and 55°C, followed by analyses of the respective adapted proteomes based on the annotated ...
Nathanael D. Arnold   +11 more
wiley   +1 more source

Process Cascade for the Production of Green Polymers from CO2 and Electric Energy

open access: yesChemie Ingenieur Technik, Volume 96, Issue 5, Page 698-712, May 2024.
A novel process cascade is introduced that combines the advantages of electrochemical CO2 conversion as CCU approach with the unrivaled synthetic potential of industrial biotechnology: CO2 is electrocatalytically reduced to formic acid which serves as substrate for the metabolically engineered bacterium Methylorubrum extorquens that prduces l‐lysine as
Jonathan Thomas Fabarius   +18 more
wiley   +1 more source

Constructing efficient bacterial cell factories to enable one‐carbon utilization based on quantitative biology: A review

open access: yesQuantitative Biology, Volume 12, Issue 1, Page 1-14, March 2024.
Abstract Developing methylotrophic cell factories that can efficiently catalyze organic one‐carbon (C1) feedstocks derived from electrocatalytic reduction of carbon dioxide into bio‐based chemicals and biofuels is of strategic significance for building a carbon‐neutral, sustainable economic and industrial system.
Yazhen Song   +9 more
wiley   +1 more source

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